Dataset Information

Protective cellular mechanism of estrogen against kidney stone formation: A proteomics approach and functional validation

ABSTRACT: Kidney stone disease is influenced by multiple factors, including but not limited to age, gender, genetic background, hydration status, diet and drug. Regarding the gender, epidemiologic data across the world has shown that females at the reproductive age (15-49 years) have lower incidence/prevalence of kidney stone disease approximately 1.5-2.5 folds as compared to males at the same age. However, this gap is narrower in the postmenopausal age, whereas the postmenopausal females with higher serum estrogen levels are less likely to have kidney stones. Furthermore, female stone formers (patients with kidney stones) are associated with lower estrogen levels. Therefore, estrogen has been proposed to serve as the protective hormone against kidney stone disease. However, the precise mechanisms underlying such protective effects of estrogen remain unclear and require further investigations. This study thus investigated the effects of estradiol (which is the most prevalent and potent form of estrogen in females at the reproductive age) on cellular proteome of renal tubular cells using a proteomics approach.

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Epithelial Cell, Mdck Cell

SUBMITTER: Visith Thongboonkerd

LAB HEAD: Prof. Visith Thongboonkerd

PROVIDER: PXD014768 | Pride | 2019-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	SN080_MDHUP00515S00696FR33.mgf	Mgf
	SN080_MDHUP00515S00696FR33.mzdata.xml	Xml
	SN080_MDHUP00515S00696FR33.mzid.gz	Mzid
	SN080_MDHUP00515S00696FR33.pride.mgf.gz	Mgf
	SN080_MDHUP00515S00696FR33.pride.mztab.gz	Mztab

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Protective Cellular Mechanism of Estrogen Against Kidney Stone Formation: A Proteomics Approach and Functional Validation.

Peerapen Paleerath P Thongboonkerd Visith V

Proteomics 20190918 19

Females have less incidence/prevalence of kidney stone disease than males. Estrogen thus may serve as the protective factor but with unclear mechanism. This study explores cellular mechanism underlying such stone preventive mechanism of estrogen. Madin darby canine kidney (MDCK) renal tubular cells are incubated with or without 20 nm 17β-estradiol for 7 days. Comparative proteomics reveals 58 differentially expressed proteins in estrogen-treated versus control cells that are successfully identif ...[more]

PMID: 31475403

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