Proteomics

Dataset Information

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Brugia malayi glycophosphatidylinositol-anchored proteome


ABSTRACT: A proteomics strategy was used to identify putative GPI-APs from adult B. malayi. Three different sample types were prepared for analysis. Firstly, intact adult worms were treated with PI-PLC to enzymatically release and solubilize the protein away from the lipid moiety. A mock-treatment with no PI-PLC was performed as a negative control. The samples derived from treatment of the intact worms have been named “Surface” but it should be noted that the proteins could originate from any exposed surface like the mouth, vagina, or rectum of the worm. Secondly, a membrane fraction of B. malayi adult female worms was prepared by ultracentrifugation of a total lysate in a sucrose buffer to separate membrane proteins from soluble proteins. This membrane fraction was also treated with PI-PLC or mock-treated without PI-PLC as a negative control. Lastly, a GPI-AP enriched sample was prepared by performing a series of organic solvent partitions to extract GPI-APs from a membrane fraction. This sample was not treated with PI-PLC. Proteins in all three samples types were digested with trypsin and the resulting peptides analyzed by LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Brugia Malayi

DISEASE(S): Filariasis

SUBMITTER: Fana Mersha  

LAB HEAD: Jeremy Michael Foster

PROVIDER: PXD014949 | Pride | 2019-11-12

REPOSITORIES: Pride

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Publications

Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi.

Mersha Fana B FB   Cortes Leslie K LK   Luck Ashley N AN   McClung Colleen M CM   Ruse Cristian I CI   Taron Christopher H CH   Foster Jeremy M JM  

PloS one 20190912 9


Further characterization of essential systems in the parasitic filarial nematode Brugia malayi is needed to better understand its biology, its interaction with its hosts, and to identify critical components that can be exploited to develop novel treatments. The production of glycophosphatidylinositol-anchored proteins (GPI-APs) is essential for eukaryotic cellular and physiological function. In addition, GPI-APs perform many important roles for cells. In this study, we characterized the B. malay  ...[more]

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