Proteomics

Dataset Information

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TMT-based study of yeast metabolism and translation under nitrogen limitation


ABSTRACT: Tandem mass tag (TMT)-based relative quantification was used to study yeast metabolism under the stepwise reduction the nitrogen content in the growth medium.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Egor Vorontsov  

LAB HEAD: Jens Nielsen

PROVIDER: PXD014962 | Pride | 2020-03-13

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2824TMTresults.xlsx Xlsx
2824TMTresults20frsProteomeDiscoverer22.pdResult Other
Fusion_180220_49.raw Raw
Fusion_180220_50.raw Raw
Fusion_180220_51.raw Raw
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Publications

Nitrogen limitation reveals large reserves in metabolic and translational capacities of yeast.

Yu Rosemary R   Campbell Kate K   Pereira Rui R   Björkeroth Johan J   Qi Qi Q   Vorontsov Egor E   Sihlbom Carina C   Nielsen Jens J  

Nature communications 20200420 1


Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and transcript content. Combining multi-omics analysis with metabolic modeling, we find that seven metabolic superpathways maintain >50% metabolic capacity in reserve, with glucose metabolism maint  ...[more]

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