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Quantifying gene expression control by growth and metabolism reveals distinct regulation of central carbon metabolism genes in yeast

ABSTRACT: Here we quantitatively describe the influence of cell growth rate and amino acid metabolic context on gene expression in the eukaryal model organism Saccharomyces cerevisiae. We show that growth rate and metabolic cues regulate ~70% of the yeast transcriptome and proteome, thereby exerting gene expression control in a global manner. We find that the growth rate-dependent differential gene expression largely reflects changing availabilities of the mRNA and protein synthesis machineries, while metabolic cues influences gene expression through the availabilities of amino acids and nucleotides. Genes in central carbon metabolism, however, are regulated independently of these global physiological controls, demonstrating distinct mechanisms to control their expression levels.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Egor Vorontsov

LAB HEAD: Jens B Nielsen

PROVIDER: PXD021218 | Pride | 2021-03-15

REPOSITORIES: pride

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			Action	DRS
	2787_Scerevisiae_Only.fasta	Fasta
	2787_Scerevisiae_UPS2.fasta	Fasta
	2963_FileList_TMT_LFQ.pdf	Pdf
	Fusion_180801_08.raw	Raw
	Fusion_180801_09.raw	Raw

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