Dataset Information

The leaves of creeping bentgrass (Agrostis stolonifera) LC−ESI−MS/MS

ABSTRACT: The global emergence of soil salinization poses a serious challenge to many countries and regions. γ-Aminobutyric acid (GABA) is involved in systemic regulation of plant adaptation to salt stress, but the underling molecular and metabolic mechanism still remains largely unknown. The elevated endogenous GABA level by exogenous application of GABA could significantly improve salt tolerance in creeping bentgrass with the enhancement of antioxidant capacity, photosynthetic characteristics, osmotic adjustment (OA), and water use efficiency. GABA strongly upregulated transcript levels of AsPPa2, AsATPaB2, AsNHX2/4/6, and AsSOS1/20 in roots involved in enhanced capacity of Na+ compartmentalization and mitigation of Na+ toxicity in cytosol. Significant downregulation of AsHKT1/4 expression could be induced by GABA in leaves in relation to maintenance of significantly lower Na+ accumulation and higher K+/Na+ ratio. GABA-depressed aquaporins (AQPs) expression and accumulation induced declines in stomatal conductance and transpiration, thereby reducing water loss in leaves during salt stress. For metabolic regulation, GABA primarily enhanced sugars and amino acids accumulation and metabolism largely contributing to improved salt tolerance through maintaining OA and metabolic homeostasis. Other major pathways could be responsible for GABA-induced salt tolerance including increases in antioxidant defense, heat shock proteins, dehydrins, and myo-inositol accumulation in leaves. Integrative analyses of molecular, protein, metabolic, and physiological changes reveal systemic function of GABA on regulating ions, water, and metabolic homeostasis in non-halophytic creeping bentgrass under salt stress.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Agrostis Stolonifera

TISSUE(S): Leaf

SUBMITTER: Zhou Li

LAB HEAD: Yan Peng

PROVIDER: PXD015066 | Pride | 2020-01-13

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	HFX2_Agrostisstolonifecal_run1_.msf	Msf
	HFX2_Agrostisstolonifecal_run1_1.raw	Raw
	HFX2_Agrostisstolonifecal_run1_10.raw	Raw
	HFX2_Agrostisstolonifecal_run1_2.raw	Raw
	HFX2_Agrostisstolonifecal_run1_3.raw	Raw

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Publications

Proteomic and Metabolomic Profilings Reveal Crucial Functions of γ-Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress.

Li Zhou Z Cheng Bizhen B Zeng Weihang W Zhang Xinquan X Peng Yan Y

Journal of proteome research 20200116 2

The global emergence of soil salinization poses a serious challenge to many countries and regions. γ-Aminobutyric acid (GABA) is involved in systemic regulation of plant adaptation to salt stress but the underlying molecular and metabolic mechanism still remains largely unknown. The elevated endogenous GABA level by the application of exogenous GABA improved salt tolerance associated with the enhancement of antioxidant capacity, photosynthetic characteristics, osmotic adjustment (OA), and water ...[more]

PMID: 31916766

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