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Development of an sulfoxide-based, CuAAC-enrichable cross-linker

ABSTRACT: Cross-linking of BSA with a novel cross-linker. Modification of the cross-linker containing peptides with CuAAC-chemistry to attach a cleavable biotin-derivative. Enrichment with streptavidin-beads.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Michael Stadlmeier

LAB HEAD: Thomas Carell

PROVIDER: PXD015080 | Pride | 2019-10-14

REPOSITORIES: Pride

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			Action	DRS
	20190719_leruch_0p23ugBSA_HSAKp_10ugHEKbackground_R1_2uL.mgf	Mgf
	20190719_leruch_0p23ugBSA_HSAKp_10ugHEKbackground_R1_2uL.raw	Raw
	20190719_leruch_0p23ugBSA_HSAKp_10ugHEKbackground_R1_2uL.zhrm	Other
	20190719_leruch_0p23ugBSA_HSAKp_10ugHEKbackground_R2_2uL.mgf	Mgf
	20190719_leruch_0p23ugBSA_HSAKp_10ugHEKbackground_R2_2uL.raw	Raw

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Publications

A Click-Chemistry-Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry.

Stadlmeier Michael M Runtsch Leander Simon LS Streshnev Filipp F Wühr Martin M Carell Thomas T

Chembiochem : a European journal of chemical biology 20191128 1-2

Mass spectrometry is the method of choice for the characterisation of proteomes. Most proteins operate in protein complexes, in which their close association modulates their function. However, with standard MS analysis, information on protein-protein interactions is lost and no structural information is retained. To gain structural and interactome data, new crosslinking reagents are needed that freeze inter- and intramolecular interactions. Herein, the development of a new reagent, which has sev ...[more]

PMID: 31593346

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Proteome profiles of primary pancreatic ductal adenocarcinomas undergoing adjuvant chemoradiation link ALDH1A1 to early local recurrence and chemoradiation resistance

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