Dataset Information

Cucumber mosaic virus resistance: Comparative proteomics of contrasting Cucumis sativus cultivars after long-term infection

ABSTRACT: Plant viruses are a major threat for a wide range of host species, causing substantial losses in agriculture. Particularly, Cucumber mosaic virus (CMV) evokes severe symptoms, thus dramatically limiting yield. Activation of plant immunity is associated with changes in the gene expression and consequently, cellular proteome to ensure virus resistance. Proteomics proved to be an extremely valuable tool for discovering multiple targets for the rational design of plant protection strategies. Herein, we studied two cultivars of cucumber (Cucumis sativus) resistant ´Heliana´ and susceptible ´Vanda´. Plant cotyledons were mechanically inoculated with CMV isolate PK1, and systemic leaves were harvested at 33 days post-inoculation. Upon protein extraction and filter-aided sample preparations, peptides were profiled by ultrahigh-performance liquid chromatography and comprehensively quantified by ion mobility enhanced mass spectrometry. From 1,516 reproducibly quantified proteins using label-free approach, 133 were differentially abundant among genotypes or treatments by strict statistic and effect size criteria. Pigments and hydrogen peroxide measurements corroborated proteomic findings. Advanced bioinformatics revealed a modular network of affected host proteins. Direct comparison of both genotypes in the uninfected state highlighted more abundant photosynthetic and development-related proteins in resistant cucumber cultivar. Long-term CMV infection showed worse preservation of energy processes and less robust translation in susceptible cultivar versus resistant genotype. Contrary, susceptible cultivar had numerous more abundant stress and defense-related proteins. We proposed promising targets for functional validation in transgenic lines a step toward durable virus resistance in cucurbits and other crops.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Cucumber Mosaic Virus Viruses Cucumis Sativus

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Maksym Danchenko

LAB HEAD: Andrej Kovac

PROVIDER: PXD015088 | Pride | 2020-01-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CMV-cucumber.DerivedFromSearchf42a3244-7f70-4034-bbc2-bb6b1366a356.mgf	Mgf
	CMV-cucumber.DerivedFromSearchf42a3244-7f70-4034-bbc2-bb6b1366a356.pride.mgf.gz	Mgf
	CMV-cucumber.mzid.gz	Mzid
	CMV-cucumber.pride.mztab.gz	Mztab
	pride.zip	Other

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Cucumber mosaic virus resistance: Comparative proteomics of contrasting Cucumis sativus cultivars after long-term infection.

Nováková Slavomíra S Šubr Zdeno Z Kováč Andrej A Fialová Ivana I Beke Gábor G Danchenko Maksym M

Journal of proteomics 20191224

Plant viruses are a significant threat to a wide range of host species, causing substantial losses in agriculture. Particularly, Cucumber mosaic virus (CMV) evokes severe symptoms, thus dramatically limiting yield. Activation of plant defense reactions is associated with changes in the cellular proteome to ensure virus resistance. Herein, we studied two cultivars of cucumber (Cucumis sativus) resistant host Heliana and susceptible host Vanda. Plant cotyledons were mechanically inoculated with CM ...[more]

PMID: 31881349

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