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Proteome profiling of mouse germinal center B cells

ABSTRACT: Productive B cell responses are critical to protect a host from infection. The spleen and lymph nodes are populated by resting follicular B cells, which can enter germinal centers upon antigen encounter. Once in the germinal center, B cells migrate between the dark and light zones, where they undergo somatic hypermutation and selection, respectively. While germinal center B cells have been studied, an intense molecular understanding of these cells/subsets (and the differences between them) is lacking.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): B Cell, Lymph Node

SUBMITTER: Hong Wang

LAB HEAD: Junmin Peng

PROVIDER: PXD015524 | Pride | 2020-04-06

REPOSITORIES: Pride

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			Action	DRS
	p01.1.pepXML	Pepxml
	p01.raw	Raw
	p02.1.pepXML	Pepxml
	p02.raw	Raw
	p03.1.pepXML	Pepxml

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Publications

Novel specialized cell state and spatial compartments within the germinal center.

Kennedy Domenick E DE Okoreeh Michael K MK Maienschein-Cline Mark M Ai Junting J Veselits Margaret M McLean Kaitlin C KC Dhungana Yogesh Y Wang Hong H Peng Junmin J Chi Hongbo H Mandal Malay M Clark Marcus R MR

Nature immunology 20200427 6

Within germinal centers (GCs), complex and highly orchestrated molecular programs must balance proliferation, somatic hypermutation and selection to both provide effective humoral immunity and to protect against genomic instability and neoplastic transformation. In contrast to this complexity, GC B cells are canonically divided into two principal populations, dark zone (DZ) and light zone (LZ) cells. We now demonstrate that, following selection in the LZ, B cells migrated to specialized sites wi ...[more]

PMID: 32341509

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