Dataset Information

Characterization of mitochondrial proteome in human langherans cells

ABSTRACT: Please provide an overall description of your study, think something similar in scope to the manuscript abstractPhysical and functional interactions between the cells and their substrate, defined as mechanotransduction, still represent a developing frontier of life sciences, despite the growing consensus for the relevance in the control of gene regulation and cell state. In this framework, the overall goal of this project was to identify and functionally characterise at the molecular level the mechanotransductive pathways, connecting the cellular microenvironment and the nucleus of Langherans cells, and to monitor the accompanying modulations in mitochondrial-related cell energetics by a proteomic approach.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Simona Nonnis

LAB HEAD: Gabriella Tedeschi

PROVIDER: PXD015739 | Pride | 2020-08-31

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	19-05-30-18P44-15nm-MITO-1.raw	Raw
	19-05-30-18P44-15nm-MITO-2.raw	Raw
	19-05-30-18P44-15nm-MITO-3.raw	Raw
	19-05-30-18P44-CTR-MITO-1.raw	Raw
	19-05-30-18P44-CTR-MITO-2.raw	Raw

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Publications

Proteomic Analysis Reveals a Mitochondrial Remodeling of βTC3 Cells in Response to Nanotopography.

Maffioli Elisa E Galli Alessandra A Nonnis Simona S Marku Algerta A Negri Armando A Piazzoni Claudio C Milani Paolo P Lenardi Cristina C Perego Carla C Tedeschi Gabriella G

Frontiers in cell and developmental biology 20200729

Recently, using cluster-assembled zirconia substrates with tailored roughness produced by supersonic cluster beam deposition, we demonstrated that β cells can sense nanoscale features of the substrate and can translate these stimuli into a mechanotransductive pathway capable of preserveing β-cell differentiation and function <i>in vitro</i> in long-term cultures of human islets. Using the same proteomic approach, we now focused on the mitochondrial fraction of βTC3 cells grown on the same zircon ...[more]

PMID: 32850772

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