Dataset Information

Label free quantitative (LFQ) proteomic analysis of mouse lymphatic endothelial cells and primary mouse dendritic cells in response to IFNgamma treatment in presence or absence of 3-HKA

ABSTRACT: To explore the cellular pathways and the molecular functions affected by a novel biogenic amine, (3-HKA), we performed a label free quantitative (LFQ) proteomic analysis of mouse lymphatic endothelial cells (LEC), and primary dendritic cells, treated with IFNgammaplus or minus 3-HKA. Biological triplicates, of total cell lysates, were fractionated by one-dimensional gel electrophoresis (1DEF) and the “in gel” tryptic derived peptides analyzed by nano-LC-ESI-MS/MS on a Q Exactive HF quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). LFQ analysis highlighted that 3-HKA downregulated many of the inflammatory pathways, more notably JAK/STAT1 and NFkB, associated with IFNgamma activation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Endothelial Cell Of Lymphatic Vessel, Lymphatic Endothelial Cell, Dendritic Cell

SUBMITTER: Cristina Clement

LAB HEAD: Laura Santambrogio

PROVIDER: PXD015865 | Pride | 2021-07-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	QEHF_20180116_LSantambrogio_S10_1.mgf	Mgf
	QEHF_20180116_LSantambrogio_S10_1.raw	Raw
	QEHF_20180116_LSantambrogio_S10_2.mgf	Mgf
	QEHF_20180116_LSantambrogio_S10_2.raw	Raw
	QEHF_20180116_LSantambrogio_S10_3.mgf	Mgf

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Publications

3-hydroxy-L-kynurenamine is an immunomodulatory biogenic amine.

Clement Cristina C CC D'Alessandro Angelo A Thangaswamy Sangeetha S Chalmers Samantha S Furtado Raquel R Spada Sheila S Mondanelli Giada G Ianni Federica F Gehrke Sarah S Gargaro Marco M Manni Giorgia G Cara Luisa Carlota Lopez LCL Runge Peter P Tsai Wanxia Li WL Karaman Sinem S Arasa Jorge J Fernandez-Rodriguez Ruben R Beck Amanda A Macchiarulo Antonio A Gadina Massimo M Halin Cornelia C Fallarino Francesca F Skobe Mihaela M Veldhoen Marc M Moretti Simone S Formenti Silvia S Demaria Sandra S Soni Rajesh K RK Galarini Roberta R Sardella Roccaldo R Lauvau Gregoire G Putterman Chaim C Alitalo Kari K Grohmann Ursula U Santambrogio Laura L

Nature communications 20210721 1

Tryptophan catabolism is a major metabolic pathway utilized by several professional and non-professional antigen presenting cells to maintain immunological tolerance. Here we report that 3-hydroxy-L-kynurenamine (3-HKA) is a biogenic amine produced via an alternative pathway of tryptophan metabolism. In vitro, 3-HKA has an anti-inflammatory profile by inhibiting the IFN-γ mediated STAT1/NF-κΒ pathway in both mouse and human dendritic cells (DCs) with a consequent decrease in the release of pro-i ...[more]

PMID: 34290243

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