Proteomics,Multiomics

Dataset Information

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Selective autophagy cargo receptor NBR1 is involved in plant response to sulfur deficit


ABSTRACT: Autophagy involvement in plant response to nitrogen, carbon and sulfur starvation was already reported, however the mechanisms responsible for its regulation and selectivity in such conditions were not yet investigated. We observed increased amounts of NBR1 transcript in plants exposed to sulfur deficit as compared to the control plants grown in nutrient sufficient conditions. This observation prompted us to investigate the role of this selective autophagy cargo receptor in plant response to sulfur deficit. Transcriptome analysis of the wild type and NBR overexpressing plants revealed differences in gene expression changes in response to sulfur deficit. Moreover, NBR1 overexpressors have significantly shorter roots than WT, when grown in nutrient deficient conditions in the presence of TOR kinase inhibitors, namely in the conditions leading to autophagy induction. Besides, NBR1 overexpression promoted stomata closure while NBR1 depletion stomata opening. Surprisingly, all lines had more closed stomata when grown in sulfur deficient than sulfur optimal conditions, what indicates that this effect is independent from NBR1. Similarly, ABA-dependent stomatal closure was independent from NBR1 and growth conditions. Cysteine also promoted stomatal closure in NBR1-independent way in plants grown in the optimal medium but in contrast, reduced the number of open stomata in plants from sulfur deficient medium. Interaction network analysis of the proteins co-purifying with NBR1 revealed links with proteins involved in degradation systems, and endosomal trafficking and a surprizing connection with nuclear transport. In addition, several proteins co-purifying with NBR1 were found only in sulfur deficient conditions. One of them, ribosomal protein S6 (RPS6) was further confirmed as a direct NBR1 interactor. Localization of the RPS6 interaction sites in NBR1 indicated that ubiquitin binding domain of NBR1 is not required for this interaction what means that it is rather not the classical “ubiquitinated target - autophagy receptor” interaction.

OTHER RELATED OMICS DATASETS IN: GSE122705

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Dominik Cysewski  

LAB HEAD: Agnieszka Sirko

PROVIDER: PXD016026 | Pride | 2020-05-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
70426688tar_WT4-S1K_1.raw Raw
70426689tar_WT4-S1L_2.raw Raw
70426690tar_WT4nS1K_3.raw Raw
70426691tar_WT4nS1L_4.raw Raw
70426692tar_WT10-S1K_5.raw Raw
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Publications

Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit.

Tarnowski Leszek L   Collados Rodriguez Milagros M   Brzywczy Jerzy J   Cysewski Dominik D   Wawrzynska Anna A   Sirko Agnieszka A  

Cells 20200310 3


Plants exposed to sulfur deficit elevate the transcription of <i>NBR1</i> what might reflect an increased demand for NBR1 in such conditions. Therefore, we investigated the role of this selective autophagy cargo receptor in plant response to sulfur deficit (-S). Transcriptome analysis of the wild type and NBR1 overexpressing plants pointed out differences in gene expression in response to -S. Our attention focused particularly on the genes upregulated by -S in roots of both lines because of sign  ...[more]

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