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Toxoplasma MYR1 Immunoprecipitations

ABSTRACT: IP-MS experiments to identify interacting proteins of Toxoplasma MYR1.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Toxoplasma Gondii Rh

TISSUE(S): Cell Culture

SUBMITTER: Alicja Cygan

LAB HEAD: John C. Boothroyd

PROVIDER: PXD016383 | Pride | 2020-02-20

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	IP1_MYR1.raw	Raw
	IP1_RH.raw	Raw
	IP2_MYR1.raw	Raw
	IP2_RH.raw	Raw
	evidence.txt	Txt

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Publications

Coimmunoprecipitation with MYR1 Identifies Three Additional Proteins within the Toxoplasma gondii Parasitophorous Vacuole Required for Translocation of Dense Granule Effectors into Host Cells.

Cygan Alicja M AM Theisen Terence C TC Mendoza Alma G AG Marino Nicole D ND Panas Michael W MW Boothroyd John C JC

mSphere 20200219 1

<i>Toxoplasma gondii</i> is a ubiquitous, intracellular protozoan that extensively modifies infected host cells through secreted effector proteins. Many such effectors must be translocated across the parasitophorous vacuole (PV), in which the parasites replicate, ultimately ending up in the host cytosol or nucleus. This translocation has previously been shown to be dependent on five parasite proteins: MYR1, MYR2, MYR3, ROP17, and ASP5. We report here the identification of several MYR1-interactin ...[more]

PMID: 32075880

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