Proteomics

Dataset Information

0

Plasma membrane anchored NAA60 N-terminal acetylation profiling in Arabidopsis thaliana

ABSTRACT: N-terminal acetylation (NTA) is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-acetyltransferases. AtNAA60 localizes to the plasma membrane in vivo by an α-helical membrane anchor at its C-terminus. In this study, we investigated the Arabidopsis thaliana N-terminal acetylome in leaf cells of naa60-1 to identify AtNAA60 substrates in vivo using the SILProNAQ approach.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Willy Bienvenut  

LAB HEAD: Willy Bienvenut

PROVIDER: PXD016494 | Pride | 2020-07-15

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20170210_WIB_DNaa60-Ech05_Frac02-05_Ch1-R.dat Other
20170210_WIB_DNaa60-Ech05_Frac02-05_Ch1-R.mgf Mgf
20170210_WIB_DNaa60-Ech05_Frac02-05_Ch1-R.raw Raw
20170210_WIB_DNaa60-Ech05_Frac02-05_Ch1.dat Other
20170210_WIB_DNaa60-Ech05_Frac02-05_Ch1.mgf Mgf
Items per page:
1 - 5 of 531
altmetric image

Publications

In humans and plants, N-terminal acetylation plays a central role in protein homeostasis, affects 80% of proteins in the cytoplasm and is catalyzed by five ribosome-associated N-acetyltransferases (NatA-E). Humans also possess a Golgi-associated NatF (HsNAA60) that is essential for Golgi integrity. Remarkably, NAA60 is absent in fungi and has not been identified in plants. Here we identify and characterize the first plasma membrane-anchored post-translationally acting N-acetyltransferase AtNAA60  ...[more]

Similar Datasets

Proteomics AtNAA50 N-terminal acetylation profiling using A. thaliana naa50 KO mutants
2020-07-15 | PXD017770 | Pride
Proteomics,Multiomics N-terminal acetylation by NatB in Arabidopsis thaliana
2019-11-21 | PXD016209 | Pride
Proteomics Global acetylation profiling for multiple Arabidopsis thaliana plastid N-terminal acetyltransferases
2020-07-13 | PXD016205 | Pride
Proteomics Global acetylation profiling for the Arabidopsis thaliana plasma membrane anchored NAA60 N-terminal acetyltransferase
2020-07-15 | PXD016533 | Pride
Proteomics Global acetylation profiling for the Arabidopsis thaliana the N-acetyltransferase NAA50 in the NatA complex.
2020-07-15 | PXD017767 | Pride
Proteomics,Multiomics Proteome imprinting by N-terminal acetylation is a vital hormone-regulated master switch during stress
2016-06-14 | PXD002069 | Pride
Proteomics Identification and characterization of the first Nα-acetyltransferase in plastids
2015-06-29 | PXD001947 | Pride
Proteomics Proteome-wide analysis of the amino terminal status of Escherichia coli proteins at the steady-state
2015-07-22 | PXD001983 | Pride
Proteomics Proteome-wide analysis of the amino terminal status of Escherichia coli proteins at the steady-state and upon deformylation inhibition
2015-07-21 | PXD002012 | Pride
Proteomics Large scale investigation of the phage Vp16 PDF activity
2017-09-25 | PXD006008 | Pride