Proteomics

Dataset Information

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AtNAA50 N-terminal acetylation profiling using A. thaliana naa50 KO mutants

ABSTRACT: N-terminal acetylation is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-terminal acetyltransferases. AtNAA50 interreact with the NatA complex (NAA10 and NAA15) to form the NatE complex. In this study, we investigated the Arabidopsis thaliana N-terminal acetylome in leaf cells with KO naa50 gene to identify in vivo specific substrates using the SILProNAQ approach.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Willy Bienvenut  

LAB HEAD: Willy Bienvenut

PROVIDER: PXD017770 | Pride | 2020-07-15

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20180518_WIB-MEB-NAA50-WT1-Fracs09-11-Ch1.dat Other
20180518_WIB-MEB-NAA50-WT1-Fracs09-11-Ch1.mgf Mgf
20180518_WIB-MEB-NAA50-WT1-Fracs09-11-Ch1.raw Raw
20180518_WIB-MEB-NAA50-WT1-Fracs09-11-Ch2.dat Other
20180518_WIB-MEB-NAA50-WT1-Fracs09-11-Ch2.mgf Mgf
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Publications

NAA50 Is an Enzymatically Active <i>N</i> <sup>α</sup>-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses.

Armbruster Laura L   Linster Eric E   Boyer Jean-Baptiste JB   Brünje Annika A   Eirich Jürgen J   Stephan Iwona I   Bienvenut Willy V WV   Weidenhausen Jonas J   Meinnel Thierry T   Hell Ruediger R   Sinning Irmgard I   Finkemeier Iris I   Giglione Carmela C   Wirtz Markus M  

Plant physiology 20200527 4

N<sup>α</sup>-terminal acetylation (NTA) is a prevalent protein modification in eukaryotes. In plants, the biological function of NTA remains enigmatic. The dominant <i>N</i>-acetyltransferase (Nat) in Arabidopsis (<i>Arabidopsis thaliana</i>) is NatA, which cotranslationally catalyzes acetylation of ∼40% of the proteome. The core NatA complex consists of the catalytic subunit NAA10 and the ribosome-anchoring subunit NAA15. In human (<i>Homo sapiens</i>), fruit fly (<i>Drosophila melanogaster</i  ...[more]

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