Proteomics

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Yeast cell cycle proteome and phosphoproteome (LC-MSMS)

ABSTRACT: Multi-omic absolute quantitative analysis of the yeast (Saccharomyces cerevisiae) mitotic cell cycle. Transcriptomics (RNA-Seq), proteomics (SILAC/ iBAQ), phosphoproteomics (SILAC/ iBAQ, enrichment with TiO2), and untargeted metabolomics (Metabolon, Inc.) were performed, all in biological triplicate. Three sub-projects from this central project were generated, in order of priority: (1) growth on glucose (n= 30 samples) (2) growth on ethanol (n= 21) and (3) pheromone effect (n= 51 samples; combined glucose and ethanol samples). For each sample, every omic type was analysed (n=4; transcriptomic, proteomic, phosphoproteomic, and metabolomic). Total omic samples generated for project = 204.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Kate Campbell  

LAB HEAD: Jens B Nielsen

PROVIDER: PXD016519 | Pride | 2020-03-26

REPOSITORIES: Pride

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161020_03_Orbi2_SK_SER_G480_T515_Chalmers_Campbell_IS1_IS3_UPS2.raw Raw
161020_05_Orbi2_SK_SER_G480_T515_Chalmers_Campbell_IS1_IS3_UPS2.raw Raw
161020_07_Orbi2_SK_SER_G480_T515_Chalmers_Campbell_IS1_IS3_UPS2.raw Raw
161104_07_Orbi2_SK_SER_G480_T515_Chalmers_Campbell_IS24_UPS2.raw Raw
161104_09_Orbi2_SK_SER_G480_T515_Chalmers_Campbell_IS24_UPS2.raw Raw
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Publications

Building blocks are synthesized on demand during the yeast cell cycle.

Campbell Kate K   Westholm Jakub J   Kasvandik Sergo S   Di Bartolomeo Francesca F   Mormino Maurizio M   Nielsen Jens J  

Proceedings of the National Academy of Sciences of the United States of America 20200325 14

For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action of metabolism and protein synthesis during progressive phases of cell division. A global understanding of which biosynthetic processes are involved and how they are temporally regulated during replication is, however, currently lacking. Here, quantitative multiomics analysis is used to generate a holistic view of the eukaryal cell cycle, using the budding yeast <i>Saccharomyces ce  ...[more]

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