Dataset Information

In-depth proteomics and natural peptidomics analyses of human endometrial fluid

ABSTRACT: Endometrial fluid reflects the status of the endometrium, being a good atraumatic source of information on embryo implantation and some pathological conditions. Although some attempts have been made to characterize it, the catalogue of the human endometrial fluid proteins remains incomplete and little has been done regarding the analysis of the containing natural peptides. In this work, we have thoroughly analyzed the protein and natural peptide content of endometrial fluid. Protein content coming from 11 different individuals was analyzed using the novel TIMS TOF Pro mass spectrometer. A total of 4694 proteins were identified with at least one peptide with FDR<1%, of which 2261 were identified in more than 50% of the samples. Besides, a pooled endometrial fluid sample was used for isolating the natural peptides and analyzed by mass spectrometry. Following this method we identified 3899 naturally-occurring peptides from 238 different proteins.

INSTRUMENT(S): LTQ Orbitrap, timsTOF Pro, Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mikel Azkargorta

LAB HEAD: Felix Elortza

PROVIDER: PXD016707 | Pride | 2020-09-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	EFAProteomics_100mingrad_Ind1.msf	Msf
	EFAProteomics_100mingrad_Ind1.mzML	Mzml
	EFAProteomics_100mingrad_Ind10.msf	Msf
	EFAProteomics_100mingrad_Ind10.mzML	Mzml
	EFAProteomics_100mingrad_Ind11.msf	Msf

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Publications

In-depth proteomics and natural peptidomics analyses reveal antibacterial peptides in human endometrial fluid.

Azkargorta Mikel M Bregón-Villahoz Marta M Escobes Iraide I Ibáñez-Pérez Jone J Iloro Ibon I Iglesias Maria M Diez-Zapirain Miren M Rabanal Aintzane A Prieto Begoña B Moragues Maria-Dolores MD Matorras Roberto R Elortza Felix F

Journal of proteomics 20200118

The composition of endometrial fluid reflects the status of the endometrium; it is a good atraumatic source of information on embryo implantation processes and possible pathological conditions. Although some attempts have been made to characterise its proteome, the catalogue of its proteins remains incomplete and little has been done to analyse the natural peptides it contains. Here, we present a comprehensive analysis of the proteins and natural peptides of the endometrial fluid. The protein co ...[more]

PMID: 31958637

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