Proteomics

Dataset Information

0

Phosphoproteomics analysis to identify PPP2R5D substrates

ABSTRACT: The purpose of this project is to identify the substrates that are specific for B'delta regulatory subunit. For this purpose, we have generated two doxycycline-inducible stable cell lines (OE and RE) that express B'delta. In the OE cell lines, B'delta is overexpressed without downregulation of the endogenous regulatory subunits. On the other hand, in the RE cell lines, B'delta is overexpressed concurrently with a downregulation of the endogenous regulatory subunits. To stimulate protein phosphorylation, we used isoproterenol, a beta-adrenergic agonist that is known to induce the PKA-mediated phosphorylation and activation of the B'd.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Laura Herring  

LAB HEAD: Stephan Strack

PROVIDER: PXD016809 | Pride | 2020-04-17

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20180926_PC494-Phospho_Fraction1_Rep1.raw Raw
20180926_PC494-Phospho_Fraction1_Rep2.raw Raw
20180926_PC494-Phospho_Fraction2_Rep1.raw Raw
20180926_PC494-Phospho_Fraction2_Rep2.raw Raw
20180926_PC494-Phospho_Fraction3_Rep1.raw Raw
Items per page:
1 - 5 of 13
altmetric image

Publications

Reduction of protein phosphatase 2A (PP2A) complexity reveals cellular functions and dephosphorylation motifs of the PP2A/B'δ holoenzyme.

Jong Chian Ju CJ   Merrill Ronald A RA   Wilkerson Emily M EM   Herring Laura E LE   Graves Lee M LM   Strack Stefan S  

The Journal of biological chemistry 20200310 17

Protein phosphatase 2A (PP2A) is a large enzyme family responsible for most cellular Ser/Thr dephosphorylation events. PP2A substrate specificity, localization, and regulation by second messengers rely on more than a dozen regulatory subunits (including B/R2, B'/R5, and B″/R3), which form the PP2A heterotrimeric holoenzyme by associating with a dimer comprising scaffolding (A) and catalytic (C) subunits. Because of partial redundancy and high endogenous expression of PP2A holoenzymes, traditiona  ...[more]

Similar Datasets

Transcriptomics Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men
2010-09-21 | E-GEOD-24215 | biostudies-arrayexpress
Proteomics SWATH-MS analysis of transgenic tobacco overexpressing 3-hydroxy-3-methylglutaryl-CoA synthase
2021-01-04 | PXD006869 | Pride
Genomics Identification of chromatin regulatory sites in conditions of basal and increased SF-1 dosage in the H295R adrenocortical tumor cell line
2013-08-01 | E-GEOD-44221 | biostudies-arrayexpress
Transcriptomics RNA-seq of the primary patient-derived GBM cell line G797 following siRNA-mediated knockdown and pcDNA3.1-Neomycin-mediated overexpression of the lncRNA HOXA10-AS
2021-10-02 | E-MTAB-10944 | biostudies-arrayexpress
Transcriptomics Fine-tuning of the anaerobic response in plants relies on trihelix protein repression of the oxygen sensing machinery
2014-07-30 | E-GEOD-50679 | biostudies-arrayexpress
Transcriptomics Ion Transporter NKCC1 - Modulator of Neurogenesis in Murine Olfactory Neurons
2015-05-07 | E-GEOD-65388 | biostudies-arrayexpress
Transcriptomics Deep sequencing of the murine olfactory receptor neuron transcriptome
2015-01-21 | E-GEOD-53793 | biostudies-arrayexpress
Transcriptomics Arabidopsis thaliana roots at 7DAG; Col-0, scl3 (LOF) and SCL3 OE (GOF)
2012-10-13 | E-GEOD-41544 | biostudies-arrayexpress
Transcriptomics Arabidopsis thaliana roots at 7DAG; ga1, ga1 scl3 (LOF) and ga1 SCL3 OE (GOF)
2014-09-05 | E-GEOD-61104 | biostudies-arrayexpress
Transcriptomics Bmp signaling regulates a dosage dependent transcriptional program to control facial skeletal development.
2011-12-17 | E-GEOD-34502 | biostudies-arrayexpress