Proteomics

Dataset Information

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Toxoplasma gondii eIF-5A knockdown iTraq


ABSTRACT: Proliferative stage (tachyzoite) is a critical factor in the transmission and pathogenesis of Toxoplasma gondii (T. gondii), which is capable of infecting a large variety of host cells. Proto-oncogene eukaryotic translation initiation factor (eIF-5A) is thought to function in regulation of many cellular processes including cell proliferation. Investigation the specific roles of eIF-5A involved in T. gondii is instrumental for the elucidation of tachyzoite replication. Here, eIF-5A knockdown strain was generated and analyzed by isobaric tags for relative and absolute quantification (iTraq), which suggested that proto-oncogene eIF-5A also acts as an essential gene to ensure proper protein synthesis during T. gondii invasion and replication.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii Rh

SUBMITTER: xiangrui Li  

LAB HEAD: Li xiangrui

PROVIDER: PXD016810 | Pride | 2021-09-09

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
F18FTSECKF0107_rep1.dat Other
P180032_1.raw Raw
P180032_10.raw Raw
P180032_11.raw Raw
P180032_12.raw Raw
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Publications

Proteomics analysis reveals that the proto-oncogene eIF-5A indirectly influences the growth, invasion and replication of Toxoplasma gondii tachyzoite.

Liu Xinchao X   Li Chunjing C   Li Xiaoyu X   Ehsan Muhammad M   Lu Mingmin M   Li Ke K   Xu Lixin L   Yan Ruofeng R   Song Xiaokai X   Li XiangRui X  

Parasites & vectors 20210526 1


<h4>Background</h4>The proliferative stage (tachyzoite) of Toxoplasma gondii (T. gondii) is critical for its transmission and pathogenesis, and a proto-oncogene eukaryotic translation initiation factor (eIF-5A) plays an important role in various cellular processes such as cell multiplication.<h4>Methods</h4>We performed a proteomic study to evaluate the specific roles of eIF-5A involved in invasion and replication of T. gondii, and both in vivo and in vitro trials using eIF-5A-interfered and wil  ...[more]

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