Dataset Information

Exposure of cells to glyphosate and formulations including glyphosate

ABSTRACT: MUTZ-3-derived cells were exposed to different substances for 24h and sampled for proteome analysis.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dendritic Cell

SUBMITTER: Fredrik Levander

LAB HEAD: Fredrik Levander

PROVIDER: PXD016859 | Pride | 2020-03-10

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	180705__Tim_Sample_13_15k_2um_New_15cmcolumn_80min_01.mgf	Mgf
	180705__Tim_Sample_13_15k_2um_New_15cmcolumn_80min_01.mgf.mzid.gz	Mzid
	180705__Tim_Sample_13_15k_2um_New_15cmcolumn_80min_01.mgf.pride.mztab.gz	Mztab
	180705__Tim_Sample_13_15k_2um_New_15cmcolumn_80min_01.pride.mgf.gz	Mgf
	180705__Tim_Sample_13_15k_2um_New_15cmcolumn_80min_01.raw	Raw

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Publications

An integrated transcriptomic- and proteomic-based approach to evaluate the human skin sensitization potential of glyphosate and its commercial agrochemical formulations.

Lindberg Tim T de Ávila Renato Ivan RI Zeller Kathrin S KS Levander Fredrik F Eriksson Dennis D Chawade Aakash A Lindstedt Malin M

Journal of proteomics 20200130

We investigated the skin sensitization hazard of glyphosate, the surfactant polyethylated tallow amine (POEA) and two commercial glyphosate-containing formulations using different omics-technologies based on a human dendritic cell (DC)-like cell line. First, the GARD™skin assay, investigating changes in the expression of 200 transcripts upon cell exposure to xenobiotics, was used for skin sensitization prediction. POEA and the formulations were classified as skin sensitizers while glyphosate alo ...[more]

PMID: 32006680

Similar Datasets

Project description:The potential health consequences of glyphosate-induced gut microbiome alterations have become a matter of intense debate. As part of a multifaceted study investigating toxicity, carcinogenicity and multigenerational effects of glyphosate and its commercial herbicide formulations, we assessed changes in bacterial and fungal populations in the caecum microbiota of rats exposed prenatally until adulthood (13 weeks after weaning) to three doses of glyphosate (0.5, 5, 50 mg/kg body weight/day), or to the formulated herbicide products Roundup Bioflow and RangerPro at the same glyphosate-equivalent doses. Caecum bacterial microbiota were evaluated by 16S rRNA sequencing whilst the fungal population was determined by ITS2 amplicon sequencing. Results showed that both fungal and bacterial diversity were affected by the Roundup formulations in a dose-dependent manner, whilst glyphosate alone significantly altered only bacterial diversity. At taxa level, a reduction in Bacteroidota abundance, marked by alterations in the levels of Alloprevotella, Prevotella and Prevotellaceae UCG-003, was concomitant to increased levels of Firmicutes (e.g., Romboutsia, Dubosiella, Eubacterium brachy group or Christensenellaceae) and Actinobacteria (e.g., Enterorhabdus, Adlercreutzia, or Asaccharobacter). Treponema and Mycoplasma also had their levels reduced by the pesticide treatments. Analysis of fungal composition indicated that the abundance of the rat gut commensal Ascomycota Kazachstania was reduced while the abundance of Gibberella, Penicillium, Claviceps, Cornuvesica, Candida, Trichoderma and Sarocladium were increased by exposure to the Roundup formulations, but not to glyphosate. Altogether, our data suggest that glyphosate and its Roundup RangerPro and Bioflow caused profound changes in caecum microbiome composition by affecting the fitness of major commensals, which in turn reduced competition and allowed opportunistic fungi to grow in the gut, in particular in animals exposed to the herbicide formulations. This further indicates that changes in gut microbiome composition might influence the long-term toxicity, carcinogenicity and multigenerational effects of glyphosate-based herbicides.