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Impact of a conjugative plasmid on plant growth-promoting Bacillus velezensis

ABSTRACT: Bacillus velezensis strain GH1-13 with a native conjugative plasmid (pBV71) is thought to be beneficial to the bacterium, although no information on its effects exists. Here we show that strain GH1-13 frequently lost the plasmid during normal growth conditions in a rich medium and changed the morphology and sensitivity to selenite and tellurite. Compared to the plasmid-cured cells, the wild-type and complemented cells exhibited multicellular behavior with the expression of conjugative type IV pili and regulatory Rap homologous genes that regulate the interconnection between conjugation and biofilm formation. Further omics-based analyses of morphogenesis, biofilm formation, and antibiotic synthesis suggest that the conjugative plasmid activates envelope stress responses in association with increased biosynthesis of extracellular polysaccharide and antibiotics for protective functions of the host during exponential phase.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bacillus Velezensis

TISSUE(S): Bacterial Protein, Endospore

DISEASE(S): Disease Free

SUBMITTER: Yong-Hak Kim

LAB HEAD: Yong-Hak Kim

PROVIDER: PXD017305 | Pride | 2021-05-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PC5-1.raw	Raw
	PC5-2.raw	Raw
	PC5_merge.mgf	Mgf
	PC5h_merge.msf	Msf
	PC8-1.raw	Raw

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