Dataset Information

CARP/Ankrd1 Interacting Proteins in mouse heart

ABSTRACT: Identification of CARP/ANKRD1 interacting proteins in heart by Co-immunoprecipitation followed by Mass-spectrometry.

INSTRUMENT(S): QSTAR

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart, Cardiac Muscle Cell

SUBMITTER: Stephan Lange

LAB HEAD: Stephan Lange

PROVIDER: PXD017426 | Pride | 2022-05-19

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Stephan_CARP.group	Other
	Stephan_CARP.wiff	Wiff
	Stephan_CARP.wiff.scan	Wiff
	Stephan_CARP_PeptideSummary.txt	Txt
	Stephan_CARP_ProteinSummary.txt	Txt

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Publications

Molecular Characterisation of Titin N2A and Its Binding of CARP Reveals a Titin/Actin Cross-linking Mechanism.

Zhou Tiankun T Fleming Jennifer R JR Lange Stephan S Hessel Anthony L AL Bogomolovas Julius J Stronczek Chiara C Grundei David D Ghassemian Majid M Biju Andrea A Börgeson Emma E Bullard Belinda B Linke Wolfgang A WA Chen Ju J Kovermann Michael M Mayans Olga O

Journal of molecular biology 20210227 9

Striated muscle responds to mechanical overload by rapidly up-regulating the expression of the cardiac ankyrin repeat protein, CARP, which then targets the sarcomere by binding to titin N2A in the I-band region. To date, the role of this interaction in the stress response of muscle remains poorly understood. Here, we characterise the molecular structure of the CARP-receptor site in titin (UN2A) and its binding of CARP. We find that titin UN2A contains a central three-helix bundle fold (ca 45 res ...[more]

PMID: 33647290

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