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Heart mitochondria from CLPP knockout Mouse Complexome Profiling experiment

ABSTRACT: A high-resolution complexome profiling analysis was performed to analyse the assembly defect of mitochondrial complex I and assess is assembly intermediates. Two biological replicates of wildtype (A_CLPP_WT_01,B_CLPP_WT_02)and knockout (C_CLPP_KO_01, D_CLPP_KO_02) were analyzed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Ulrich Brandt

LAB HEAD: Ulrich Brandt

PROVIDER: PXD017614 | Pride | 2020-03-05

REPOSITORIES: Pride

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			Action	DRS
	20180425_C_CLPP_KO_01_NT_SG01.raw	Raw
	20180425_C_CLPP_KO_01_NT_SG02.raw	Raw
	20180425_C_CLPP_KO_01_NT_SG03.raw	Raw
	20180425_C_CLPP_KO_01_NT_SG04.raw	Raw
	20180425_C_CLPP_KO_01_NT_SG05.raw	Raw

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Publications

A salvage pathway maintains highly functional respiratory complex I.

Szczepanowska Karolina K Senft Katharina K Heidler Juliana J Herholz Marija M Kukat Alexandra A Höhne Michaela Nicole MN Hofsetz Eduard E Becker Christina C Kaspar Sophie S Giese Heiko H Zwicker Klaus K Guerrero-Castillo Sergio S Baumann Linda L Kauppila Johanna J Rumyantseva Anastasia A Müller Stefan S Frese Christian K CK Brandt Ulrich U Riemer Jan J Wittig Ilka I Trifunovic Aleksandra A

Nature communications 20200402 1

Regulation of the turnover of complex I (CI), the largest mitochondrial respiratory chain complex, remains enigmatic despite huge advancement in understanding its structure and the assembly. Here, we report that the NADH-oxidizing N-module of CI is turned over at a higher rate and largely independently of the rest of the complex by mitochondrial matrix protease ClpXP, which selectively removes and degrades damaged subunits. The observed mechanism seems to be a safeguard against the accumulation ...[more]

PMID: 32242014

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Complexome profiling of heart mitochondria from WT and ClpP-/- mice

Project description:Mutations in the gene for the mitochondrial matrix protease CLPP can cause human Perrault syndrome, which is characterized by male and female infertility, progressive sensorineural deafness, ataxia and leukoencephalopathy. This gene encodes a peptidase that is conserved since bacteria and localizes to mitochondrial matrix in eukaryotes. To assess the assembly and stability of mitochondrial complexes heart tissue of WT and ClpP-/- mice was dissected and enriched mitochondrial fraction was used for complexome profiling.

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