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A strain-specific genomic island encodes regulatory proteins to activate type VI secretion system expression in the squid symbiont Vibrio fischeri.

ABSTRACT: We used the bioluminescent squid symbiont, Vibrio fischeri, to identify essential regulatory factors that control expression and function of a strain-specific T6SS encoded within a genomic island. Random transposon mutagenesis revealed that three genes located on the T6SS2-encoding genomic island are necessary to activate expression of a T6SS reporter. We used a proteomics approach to identify proteins that were differentially abundant in mutant strains compared to the wild type when cells were grown in a high-viscosity media.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Aliivibrio Fischeri

SUBMITTER: Fernanda Salvato

LAB HEAD: Manuel Kleiner

PROVIDER: PXD017722 | Pride | 2021-08-24

REPOSITORIES: pride

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			Action	DRS
	1A_400ng_run2.msf	Msf
	1A_400ng_run2.pep.xml	Pepxml
	1A_400ng_run2.raw	Raw
	1B_400ng_run2.msf	Msf
	1B_400ng_run2.pep.xml	Pepxml

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Publications

Activation of the Type VI Secretion System in the Squid Symbiont <i>Vibrio fischeri</i> Requires the Transcriptional Regulator TasR and the Structural Proteins TssM and TssA.

Smith Stephanie S Salvato Fernanda F Garikipati Aditi A Kleiner Manuel M Septer Alecia N AN

Journal of bacteriology 20210809 21

Bacteria have evolved diverse strategies to compete for a niche, including the type VI secretion system (T6SS), a contact-dependent killing mechanism. T6SSs are common in bacterial pathogens, commensals, and beneficial symbionts, where they affect the diversity and spatial structure of host-associated microbial communities. Although T6SS gene clusters are often located on genomic islands (GIs), which may be transferred as a unit, the regulatory strategies that promote gene expression once the T6 ...[more]

PMID: 34370559

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