Proteomics

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Environmental viscosity modulates interbacterial killing during habitat transition

ABSTRACT: In this study we employed a proteomics method to determine how bacteria respond at the protein level to a transition from a low- to a high-viscosity liquid environment. We applied this method to explore how the symbiotic marine bacterium Vibrio fischeri responds to changes in environmental viscosity associated with the transition from free-living to host-associated states. For this project cultures of ES401 were initially grown in low-viscosity liquid media and then transferred to either fresh low- or high-viscosity liquid media, which was generated by supplementing liquid media with 5% polyvinylpyrrolidone (w/vol). Samples were then incubated for one, 12, or 24 hours, and then collected by centrifugation and cell pellets frozen at -80°C. For each treatment four biological replicates were generated and analyzed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Aliivibrio Fischeri

SUBMITTER: Fernanda Salvato  

LAB HEAD: Manuel kleiner

PROVIDER: PXD015403 | Pride | 2020-01-07

REPOSITORIES: pride

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L-L_12h_3A_400ng_run1.msf Msf
L-L_12h_3A_400ng_run1.pep.xml Pepxml
L-L_12h_3A_400ng_run1.raw Raw
L_L_12h_1A_400ng_run1.msf Msf
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Environmental Viscosity Modulates Interbacterial Killing during Habitat Transition.

Speare Lauren L   Smith Stephanie S   Salvato Fernanda F   Kleiner Manuel M   Septer Alecia N AN  

mBio 20200204 1

Symbiotic bacteria use diverse strategies to compete for host colonization sites. However, little is known about the environmental cues that modulate interbacterial competition as they transition between free-living and host-associated lifestyles. We used the mutualistic relationship between <i>Eupyrmna scolopes</i> squid and <i>Vibrio fischeri</i> bacteria to investigate how intraspecific competition is regulated as symbionts move from the seawater to a host-like environment. We recently report  ...[more]

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