Dataset Information

Glycosylation analysis of pgl enzyme from C fetus in ecoli

ABSTRACT: Analysis of purified glycoproteins to assess the impact of pgl enzymes on the construction of the C.fetus N-linked glycan

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Campylobacter Jejuni

SUBMITTER: Nichollas Scott

LAB HEAD: Christine Szymanski

PROVIDER: PXD017832 | Pride | 2020-05-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Ecoli_glycosylation_analysis.zip	Other
	Nsco_2020205_CS_gel_B2.raw	Raw
	Nsco_2020205_CS_gel_B3.raw	Raw
	Nsco_2020205_CS_gel_B4.raw	Raw
	Nsco_2020205_CS_gel_B5.raw	Raw

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Publications

Influence of Protein Glycosylation on Campylobacter fetus Physiology.

Duma Justin J Nothaft Harald H Weaver Danielle D Fodor Christopher C Beadle Bernadette B Linton Dennis D Benoit Stéphane L SL Scott Nichollas E NE Maier Robert J RJ Szymanski Christine M CM

Frontiers in microbiology 20200617

Campylobacter fetus is commonly associated with venereal disease and abortions in cattle and sheep, and can also cause intestinal or systemic infections in humans that are immunocompromised, elderly, or exposed to infected livestock. It is also believed that C. fetus infection can result from the consumption or handling of contaminated food products, but C. fetus is rarely detected in food since isolation methods are not suited for its detection and the physiology of the org ...[more]

PMID: 32625174

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Project description:Campylobacter jejuni is currently the leading cause of bacterial gastroenteritis in humans. Comparison of multiple Campylobacter strains revealed a high genetic and phenotypic diversity. However, little is known about differences in transcriptome organization, gene expression, and small RNA (sRNA) repertoires. Here we present the first comparative primary transcriptome analysis based on the differential RNAM-bM-^@M-^Sseq (dRNAM-bM-^@M-^Sseq) of four C. jejuni isolates. Our approach includes a novel, generic method for the automated annotation of transcriptional start sites (TSS), which allowed us to provide genome-wide promoter maps in the analyzed strains. These global TSS maps are refined through the integration of a SuperGenome approach that allows for a comparative TSS annotation by mapping RNAM-bM-^@M-^Sseq data of multiple strains into a common coordinate system derived from a whole-genome alignment. Considering the steadily increasing amount of RNAM-bM-^@M-^Sseq studies, our automated TSS annotation will not only facilitate transcriptome annotation for a wider range of pro- and eukaryotes but can also be adapted for the analysis among different growth or stress conditions. Our comparative dRNAM-bM-^@M-^Sseq analysis revealed conservation of most TSS, but also single-nucleotide-polymorphisms (SNP) in promoter regions, which lead to strain-specific transcriptional output. Furthermore, we identified strain-specific sRNA repertoires that could contribute to differential gene regulation among strains. In addition, we identified a novel minimal CRISPR-system in Campylobacter of the type-II CRISPR subtype, which relies on the host factor RNase III and a trans-encoded sRNA for maturation of crRNAs. This minimal system of Campylobacter, which seems active in only some strains, employs a unique maturation pathway, since the crRNAs are transcribed from individual promoters in the upstream repeats and thereby minimize the requirements for the maturation machinery. Overall, our study provides new insights into strain-specific transcriptome organization and sRNAs, and reveals genes that could modulate phenotypic variation among strains despite high conservation at the DNA level. Our dRNA-seq study of multiple C. jejuni strains represents the first comparative analysis of the primary transcriptomes of multiple strains and provides new insights into riboregulation in this bacterial pathogen.

Dataset Information

Glycosylation analysis of pgl enzyme from C fetus in ecoli

Dataset's files

Publications

Influence of Protein Glycosylation on <i>Campylobacter fetus</i> Physiology.

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