Dataset Information

Separation of the Bioactive Exosomes from Extracellular Vesicles Derived from Murine CTLs

ABSTRACT: Recently, instead of ultracentrifugation, development of new preparation protocol is demanded for research of reliable bioactivity and drug discovery of extracellular vesicles (EVs). In this study, we propose a novel method for large scale preparation of high-performance extracellular vesicles focusing on membrane negative charge.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell

SUBMITTER: Naohiro Seo

LAB HEAD: Naohiro Seo

PROVIDER: PXD017850 | Pride | 2022-04-04

REPOSITORIES: Pride

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Publications

Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.

Seo Naohiro N Nakamura Junko J Kaneda Tsuguhiro T Tateno Hiroaki H Shimoda Asako A Ichiki Takanori T Furukawa Koichi K Hirabayashi Jun J Akiyoshi Kazunari K Shiku Hiroshi H

Journal of extracellular vesicles 20220301 3

The development of a new large-scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high-performance exosomes (EXOs) by using an anion-exchange method. Cytotoxic T-lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut-off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at ...[more]

PMID: 35289089

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