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Scophthalmus maximus spleen iTRAQ

ABSTRACT: Scophthalmus maximus spleen proteome after polyI:C-injection under high or low temperature were compared by iTRAQ(isobaric tags for relative and absolute quantitation).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Scophthalmus Maximus

TISSUE(S): Spleen

SUBMITTER: Xin Yue

LAB HEAD: Baozhong,Liu

PROVIDER: PXD005293 | Pride | 2018-02-07

REPOSITORIES: Pride

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			Action	DRS
	F13FTSECKF1393_2_rep1.dat	Other
	MS141004-2-1.raw	Raw
	MS141004-2-10.raw	Raw
	MS141004-2-11.raw	Raw
	MS141004-2-12.raw	Raw

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1 - 5 of 21

Publications

Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus.

Yue Xin X Huan Pin P Hu Yonghua Y Liu Baozhong B

Scientific reports 20180130 1

A worldwide increase in the reports of diseases affecting marine organisms has paralleled the climate warming over the past few decades. In this study, we applied omics to explore the mechanisms underlying thermo-linked epizootics, by comparing both the transcriptome- and proteome-wide response of turbots to a mimic pathogen (poly I:C) between high temperature and low temperature using a time-course approach. Our results showed that myeloperoxidase (MPO) and insulin were differentially expressed ...[more]

PMID: 29382883

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Dendrobium officinale seed symbiotic germination iTRAQ

Project description:Mycorrhizal fungi colonize orchid seed and induce the germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchids. However, the molecular changes taking place during the orchid seed symbiotic germination still remains largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed comparative transcriptomic and proteomic analysis on Chinese traditional medicinal orchid plants, Dendrobium officinale to explore protein expression change at the different developmental stages between asymbiotic and symbiotic germination and identify the key proteins regulated symbiotic germination of orchid seeds. iTRAQ analysis from 8 samples identified 2256 plant proteins, of which, 308 proteins were differentially expressed across three developmental stages within asymbiotic or symbiotic accession and 229 proteins are differentially expressed in the symbiotic germination compared to asymbiotic germination. 32 proteins are co-upregulated in both proteomic and transcriptomic level for symbiotic germination compared to asymbiotic germination. Our results revealed that symbiotic germination of D. officinale seeds probably shares the common signal pathway with asymbiotic germination during the early germination stage.

2017-05-12 | PXD006102 | Pride

Liver proteome response of turbot to lysine and leucine in free and dipeptide forms

Project description:In this iTRAQ quantification project, there were 6 Scophthalmus_maximus samples named CAA-1, CAA-2, CAA-3, Lys-Leu-1, Lys-Leu-2, Lys-Leu-3 and we did one technical duplicate experiments. Totally 935928 spectrums were generated, 32261 peptides and 5708 proteins were identified with 1% false discovery rate (FDR).

2021-06-22 | PXD025276 | Pride

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in storability

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2021-09-25 | PXD025860 | Pride

Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology

Project description:Transplantation is currently the best treatment option for end-stage renal disease (ESRD) patients. However, acute rejection (AR) is the main source of failure in renal transplantation. The current gold standard for diagnosis of AR involves renal biopsy, but it is invasive, time consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve the allograft function. Here, we applied isobaric tags for relative and absolute quantisation (iTRAQ) mass spectrometry to analyse serum protein expression in AR patients and healthy controls. Overall, 1399 proteins were identified. Using a cut-off of Q<0.05 and a fold change > 1.2 for the expressed variation, 109 proteins showed distinct differential expression between the AR and control groups, of whom 72 were upregulated and 37 were downregulated. Several proteins, such as properdin, keratin 1, lipoprotein (a) and vitamin D-binding protein, may play roles in the pathogenesis of AR. This study focused on iTRAQ-based proteomic profiling of serum samples in AR. Insights from our work might help advance our understanding of the molecular mechanisms of AR and identify potential novel biomarkers of AR for further characterization.

2021-09-08 | PXD015336 | Pride

iTRAQ-based quantitative proteomic analysis of Takifugu fasciatus liver in response to low temperature stress

Project description:The effects of temperature on teleosts have been of strong interest for a long time, particularly because low temperature profoundly influences the physiological and behavioral processes of ectotherms, especially teleosts. However, the cold-tolerance characteristics of fish at a protein level remains unclear. Therefore, in order to shed further light on the molecular mechanisms of low temperature adaptation in fish, we conducted quantitative proteomics on T. fasciatus liver using iTRAQ. Comparing the proteomic profiles of the T. fasciatus liver at 12℃ and 26℃, a total of 3741 proteins were identified, and 160 were differentially expressed. Among the differentially expressed proteins, the most significant changes were noted in those involved in oxidative stress (nine proteins), mitochondrial enzymes (eleven proteins) and signal transduction (thirteen proteins). The KEGG enrichment analysis indicated significant enhancement of D-arginine and D-ornithine metabolism, MAPK signaling, Wnt signaling and Gap junction pathway. Subsequently, three significantly up-regulated (CIRB, HSP90 and GST) and two significantly down-regulated proteins (FLNB and A2ML1) were validated by the parallel reaction monitoring (PRM) assays. Furthermore, the changes in expression of proteins involved in the oxidative stress, mitochondrial enzymes and signal transduction were validated at the transcriptional level using qPCR. These verification results show that the experimental data of iTRAQ are reliable. Our results not only deepen an understanding of the mechanisms underlying low temperature tolerance in fish, but also may contribute to its breeding for enhancement of cold tolerance.

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SPD1590_iTRAQ - A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties

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