Dataset Information

Drosophila melanogaster eye Proteomics

ABSTRACT: Absolute (molar) quantification determines proteins stoichiometry in complexes, networks and metabolic pathways. We employed MS Western workflow to determine molar abundances of proteins critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric 264 kDa protein standard that covers, in total, 197 peptides from 43 proteins. Each protein was independently quantified with 2 to 4 proteotypic peptides with the coefficient of variation of less than 15 %, better than 1000-fold dynamic range and sub-femtomole sensitivity. We determined molar abundances and stoichiometric ratios of the components of the PT machinery and the rhabdomere, and how they are changing when rhabdomere morphogenesis is perturbed by genetic manipulation of the evolutionary conserved gene crumbs (crb).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Bharath Kumar Raghuraman

LAB HEAD: Andrej Shevchenko

PROVIDER: PXD018001 | Pride | 2020-07-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170301_Saritacrb_1stRound_6_0_GS_01_D3012_BSA_1pm_23ul_Dionex_120min_01.msf	Msf
	20170301_Saritacrb_1stRound_6_0_GS_01_D3012_BSA_1pm_23ul_Dionex_120min_01.raw	Raw
	20170301_Saritacrb_1stRound_6_0_GS_01_D3012_BSA_1pm_23ul_Dionex_120min_02.raw	Raw
	20170301_Saritacrb_1stRound_6_0_GS_02_D3012_BSA_1pm_23ul_Dionex_120min_01.raw	Raw
	20170301_Saritacrb_1stRound_6_0_GS_02_D3012_BSA_1pm_23ul_Dionex_120min_02.raw	Raw

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Publications

Absolute Quantification of Proteins in the Eye of Drosophila melanogaster.

Raghuraman Bharath Kumar BK Hebbar Sarita S Kumar Mukesh M Moon HongKee H Henry Ian I Knust Elisabeth E Shevchenko Andrej A

Proteomics 20200903 23

Absolute (molar) quantification of proteins determines their molar ratios in complexes, networks, and metabolic pathways. MS Western workflow is employed to determine molar abundances of proteins potentially critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric 264 kDa protein standard that covers, in total, 197 peptides from 43 proteins. The majority of proteins are independently quantified with two to four proteotypic peptides with the ...[more]

PMID: 32663363

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