Dataset Information

Eye proteome of Drosophila melanogaster

ABSTRACT: We report the proteome composition of the Drosophila eye – a compound organ that is highly enriched in membrane proteins. The fly eye is a popular model to study the physiology of vision by means of genetic, pharmacological, and dietary interference.While the eye transcriptome and development-related changes of gene expression profiles have been extensively studied, little is known about the eye proteome.we employed GeLC-MS/MS to identify and rank the abundances of 3516 eye proteins. Moreover, we applied our MS Western method to determine the absolute (molar) abundances of a related set of proteins that are important for photoreceptor structure (including maintenance) and function (phototransduction). Altogether, we provide a comprehensive and expandable proteomics resource that will be valuable for a variety of studies of ocular biochemistry, physiology, and development.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Compound Eye

SUBMITTER: Mukesh Kumar

LAB HEAD: Andrej Shevchenko

PROVIDER: PXD044845 | Pride | 2024-08-09

REPOSITORIES: Pride

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			Action	DRS
	20160618_DmDiet_3rdRound_BR1_NF_GS_01_D3012_BSA_1pm_23ul_Dionex_120min_01.raw	Raw
	20160618_DmDiet_3rdRound_BR1_NF_GS_01_D3012_BSA_1pm_23ul_Dionex_120min_02.raw	Raw
	20160618_DmDiet_3rdRound_BR1_NF_GS_02_D3012_BSA_1pm_23ul_Dionex_120min_01.raw	Raw
	20160618_DmDiet_3rdRound_BR1_NF_GS_02_D3012_BSA_1pm_23ul_Dionex_120min_02.raw	Raw
	20160618_DmDiet_3rdRound_BR1_NF_GS_03_D3012_BSA_1pm_23ul_Dionex_120min_01.raw	Raw

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Publications

Eye proteome of Drosophila melanogaster.

Kumar Mukesh M Has Canan C Lam-Kamath Khanh K Ayciriex Sophie S Dewett Deepshe D Bashir Mhamed M Poupault Clara C Schuhmann Kai K Thomas Henrik H Knittelfelder Oskar O Raghuraman Bharath Kumar BK Ahrends Robert R Rister Jens J Shevchenko Andrej A

Proteomics 20231114 10

Drosophila melanogaster is a popular model organism to elucidate the molecular mechanisms that underlie the structure and function of the eye as well as the causes of retinopathies, aging, light-induced damage, or dietary deficiencies. Large-scale screens have isolated genes whose mutation causes morphological and functional ocular defects, which led to the discovery of key components of the phototransduction cascade. However, the proteome of the Drosophila eye is poorly characterized. Here, we ...[more]

PMID: 37963819

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