Dataset Information

Tissue- and plasma-derived exosomal protein biomarkers define multiple human cancers

ABSTRACT: There is an unmet clinical need for improved tissue and liquid biopsy tools for cancer detection. We investigated the proteomic profile of exosomes in 426 human samples from tissue explants (TE), plasma and other bodily fluids. Among traditional exosome markers, CD9, HSPA8, ALIX, HSP90AB1 represent pan-exosomes/exomeres. Moreover, we identified novel pan-exosome/exomere markers (e.g., ACTB, MSN, RAP1B). As proof of principle that exosomes are ideal diagnostic tools, we analyzed the proteome of TE exosomes (n=151) or plasma-derived exosomes (n=120). Comparison of TE exosomes identified proteins (e.g., VCAN, TNC, THBS2) that distinguish tumors from normal tissues with 90% sensitivity/94% specificity. Machinelearning classification of plasma-derived exosomes revealed 95% sensitivity/90% specificity in identifying cancer-associated exosomes. Finally, we defined a panel of tumor-type specific exosomal proteins in TE and plasma, which may help classify tumors of unknown primary origin. Overall, exosomal proteins can serve as reliable biomarkers for cancer detection and for determining cancer type

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive Plus, Q Exactive HF, Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Blood-lymph, Brain, Blood Plasma, Lung, Liver, Lymph Node, Kidney, Cell Culture, Blood Serum, Muscle, Skin, Colon, Pancreas, Bile Duct, Bone Marrow, Bile

DISEASE(S): Stomach Cancer,Prostate Adenocarcinoma,Uveal Melanoma,Embryonal Rhabdomyosarcoma,Melanoma,Renal Wilms' Tumor,Fibrosarcoma,Malignant Mesothelioma,Pancreatic Cancer,Neuroblastoma,Chronic Bronchitis,Synovial Sarcoma,Peripheral Primitive Neuroectodermal Tumor,Childhood Extraosseous Osteosarcoma,Acute Leukemia,Colon Cancer,Head And Neck Cancer,Brain Cancer,Retinoblastoma,Lung Carcinoma,Cholangiocarcinoma

SUBMITTER: henrik molina

LAB HEAD: David C. Lyden

PROVIDER: PXD018301 | Pride | 2020-08-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Human512Reports.xlsx	Xlsx
	MS13375_Bruno_Lyden_MCF7_bone_A.raw	Raw
	MS13375_Bruno_Lyden_MCF7_bone_B.raw	Raw
	MS13375_Bruno_Lyden_MCF7_bone_c.raw	Raw
	MS13451QE_Bruno_Lyden_831brain_A.raw	Raw

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Publications

Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.

Hoshino Ayuko A Kim Han Sang HS Bojmar Linda L Gyan Kofi Ennu KE Cioffi Michele M Hernandez Jonathan J Zambirinis Constantinos P CP Rodrigues Gonçalo G Molina Henrik H Heissel Søren S Mark Milica Tesic MT Steiner Loïc L Benito-Martin Alberto A Lucotti Serena S Di Giannatale Angela A Offer Katharine K Nakajima Miho M Williams Caitlin C Nogués Laura L Pelissier Vatter Fanny A FA Hashimoto Ayako A Davies Alexander E AE Freitas Daniela D Kenific Candia M CM Ararso Yonathan Y Buehring Weston W Lauritzen Pernille P Ogitani Yusuke Y Sugiura Kei K Takahashi Naoko N Alečković Maša M Bailey Kayleen A KA Jolissant Joshua S JS Wang Huajuan H Harris Ashton A Schaeffer L Miles LM García-Santos Guillermo G Posner Zoe Z Balachandran Vinod P VP Khakoo Yasmin Y Raju G Praveen GP Scherz Avigdor A Sagi Irit I Scherz-Shouval Ruth R Yarden Yosef Y Oren Moshe M Malladi Mahathi M Petriccione Mary M De Braganca Kevin C KC Donzelli Maria M Fischer Cheryl C Vitolano Stephanie S Wright Geraldine P GP Ganshaw Lee L Marrano Mariel M Ahmed Amina A DeStefano Joe J Danzer Enrico E Roehrl Michael H A MHA Lacayo Norman J NJ Vincent Theresa C TC Weiser Martin R MR Brady Mary S MS Meyers Paul A PA Wexler Leonard H LH Ambati Srikanth R SR Chou Alexander J AJ Slotkin Emily K EK Modak Shakeel S Roberts Stephen S SS Basu Ellen M EM Diolaiti Daniel D Krantz Benjamin A BA Cardoso Fatima F Simpson Amber L AL Berger Michael M Rudin Charles M CM Simeone Diane M DM Jain Maneesh M Ghajar Cyrus M CM Batra Surinder K SK Stanger Ben Z BZ Bui Jack J Brown Kristy A KA Rajasekhar Vinagolu K VK Healey John H JH de Sousa Maria M Kramer Kim K Sheth Sujit S Baisch Jeanine J Pascual Virginia V Heaton Todd E TE La Quaglia Michael P MP Pisapia David J DJ Schwartz Robert R Zhang Haiying H Liu Yuan Y Shukla Arti A Blavier Laurence L DeClerck Yves A YA LaBarge Mark M Bissell Mina J MJ Caffrey Thomas C TC Grandgenett Paul M PM Hollingsworth Michael A MA Bromberg Jacqueline J Costa-Silva Bruno B Peinado Hector H Kang Yibin Y Garcia Benjamin A BA O'Reilly Eileen M EM Kelsen David D Trippett Tanya M TM Jones David R DR Matei Irina R IR Jarnagin William R WR Lyden David D

Cell 20200813 4

There is an unmet clinical need for improved tissue and liquid biopsy tools for cancer detection. We investigated the proteomic profile of extracellular vesicles and particles (EVPs) in 426 human samples from tissue explants (TEs), plasma, and other bodily fluids. Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers. To confirm that EVPs are ideal diagnostic tools, we analyzed proteomes of TE- (n = 151) ...[more]

PMID: 32795414

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