Distinct proteome profiles of Agrobacterium tumefaciens grown in bioreactors suggests bacterial adaptability and preparedness for plant transformation in molecular farming

ABSTRACT: Plants transformed via agroinfiltration using a modified strain of Agrobacterium tumefaciens can produce antibody proteins for purification and application in antibody-based therapeutics. The rising demand for such drugs underscores the expansion of molecular farming and warrants the need to produce transformed plants at an industrial scale, requiring large quantities of A. tumefaciens culture, which is not feasible using traditional growth methods (e.g., shake flask). To overcome this limitation, we investigate the use of a bioreactor growth chamber system to meet production demands through growth optimization and efficient plant transformation. We observe differences in target antibody production associated with different growth conditions and aim to characterize the unique responses through profiling of protein-level changes specific to each condition. Our quantitative proteomic profiling defines growth-specific alterations in protein abundance and highlights the functional roles of these proteins. Moreover, we observe changes in the bacterial proteomes, which may influence bacterial processes important for effective agroinfiltration and transformation. Overall, our study provides insight into fundamental biological processes of A. tumefaciens influenced by growth conditions, which may influence plant transformation efficiency and outcome of antibody-based therapeutic production.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rhizobium Radiobacter (agrobacterium Tumefaciens) (agrobacterium Radiobacter)

SUBMITTER: Jennifer Geddes-McAlister  

LAB HEAD: Jennifer Geddes-McAlister

PROVIDER: PXD018384 | Pride | 2020-12-10

REPOSITORIES: Pride