Temporal proteomic profiling of Agrobacterium tumefaciens exposure to agroinfiltration media influences cellular remodeling and may promote enhanced adaptability for molecular farming

ABSTRACT: Plants transformed via agroinfiltration using a modified strain of Agrobacterium tumefaciens can produce antibody proteins for purification and application in antibody-based therapeutics. The preparation of large quantities of plants for transformation may require bacterial exposure to agroinfiltration media, devoid of nutrients and carbon sources, for prolonged periods of time (e.g., 2 to 24 h). Such conditions may influence the viability of the bacteria and the subsequent levels of infection and target protein production. Here, we draw upon our previous work profiling growth-specific differences of A. tumefaciens associated with shake flask vs. bioreactor growth and explore the role of timing in bacterial culture preparation for agroinfiltration. We use state-of-the-art mass spectrometry-based proteomics to define processes of cellular remodeling over the time course of incubation in agroinfiltration media and detect proteins with significant changes in abundance. We observe distinct proteome profiles associated with bacterial growth conditions and exposure timing, as well as a progression towards increased cellular remodelling over time. In addition, we define unique functional characteristics influencing pathogenesis and response to stimulus, as well as changes in bacterial nutrient acquisition and adaptability. Moreover, we validate our findings with in-depth profiling of specific proteins over time and demonstrates an overlap >80% between our proteomics platforms. Overall, our results suggest that prolonged exposure of A. tumefaciens to agroinfiltration media may increase pathogenesis and motility, leading to enhanced infectivity of plant cells and production of the target drug.

OTHER RELATED OMICS DATASETS IN: MODEL1109130000 MODEL1909260003 MODEL1909260004 MODEL1703310000 MODEL1909260005 MODEL1909260006 MODEL1311110000 MODEL1311110001

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rhizobium Radiobacter (agrobacterium Tumefaciens) (agrobacterium Radiobacter)

SUBMITTER: Jennifer Geddes-McAlister  

LAB HEAD: Jennifer Geddes-McAlister

PROVIDER: PXD018405 | Pride | 2020-12-10

REPOSITORIES: Pride