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Early cleavage of preimplantation embryos is regulated by tRNAGln-TTG-derived small RNAs present in mature spermatozoa

ABSTRACT: Sperm-derived tsRNAs could act as acquired epigenetic factors and contribute to offspring phenotypes. However, the roles of specific tsRNAs in early embryo development remain to be elucidated. Here, by using pigs as a research model, we probed the tsRNA dynamics during spermatogenesis and sperm maturation, and demonstrated the delivery of tsRNAs from semen-derived exosomes to spermatozoa. By microinjection of the antisense sequence into in vitro fertilized oocytes and subsequent single-cell RNA-sequencing of embryos, we identified a specific functional tsRNA group (Gln-TTGs) that participate in the early cleavage of porcine preimplantation embryos, probably by regulating cell cycle-associated genes. Thus, specific tsRNAs present in mature spermatozoa play significant roles during preimplantation embryo development.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Oocyte

SUBMITTER: Xiaoxu Chen

LAB HEAD: Wenxian Zeng

PROVIDER: PXD019054 | Pride | 2020-07-13

REPOSITORIES: Pride

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			Action	DRS
	R20180500639_ANTI_PULL_DOWN.mgf	Mgf
	R20180500639_ANTI_PULL_DOWN.raw	Raw
	R20180500639_NC_PULL_DOWN.mgf	Mgf
	R20180500639_NC_PULL_DOWN.raw	Raw
	R20180500639_TSRNA_PULL_DOWN.mgf	Mgf

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