Dataset Information

Identification of human FGF9 IRES interacting proteins

ABSTRACT: We have previously shown that FGF9 is overexpressed in hypoxia through the IRES located in the 5’UTR. To identify the protein that binds to FGF9 IRES and controls FGF9 protein synthesis in hypoxia, FGF9 IRES RNA was in vitro synthesized and used to pulled-down interacting proteins. The RNA-protein complexes were first visualized by sliver staining, followed by cutting specific bands for protein identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).

INSTRUMENT(S): ultraflex

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: H. Sunny Sun

LAB HEAD: H. Sunny Sun

PROVIDER: PXD012892 | Pride | 2019-11-13

REPOSITORIES: Pride

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Publications

hnRNPM induces translation switch under hypoxia to promote colon cancer development.

Chen Tsung-Ming TM Lai Ming-Chih MC Li Yi-Han YH Chan Ya-Ling YL Wu Chih-Hao CH Wang Yu-Ming YM Chien Chun-Wei CW Huang San-Yuan SY Sun H Sunny HS Tsai Shaw-Jenq SJ

EBioMedicine 20190307

<h4>Background</h4>Hypoxia suppresses global protein production, yet certain essential proteins are translated through alternative pathways to survive under hypoxic stress. Translation via the internal ribosome entry site (IRES) is a means to produce proteins under stress conditions such as hypoxia; however, the underlying mechanism remains largely uncharacterized.<h4>Methods</h4>Proteomic and bioinformatic analyses were employed to identify hnRNPM as an IRES interacting factor. Clinical specime ...[more]

PMID: 30852162

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