Proteomics

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Protein components of infective endocarditis vegetations and insights on their proteolysis using mass spectrometry-based N-terminomics

ABSTRACT: Infective endocarditis results in the growth of a vegetative mass on native or bio prosthetic valves hindering function and increasing risk of thromboses. This study set out to determine the proteomic composition of these vegetations including the influence of different micro-organisms and the proteases known to be present. Our data has allowed us to describe for first time the influence different infectious organisms have on vegetation growth. Including the contribution of the immune response and circulatory proteins/cells make in composing a vegetation. Furthermore, we describe the protease activity and both known and novel cleavage sites in a plethora of targets. This data provides a deep insight into the homogeneity and heterogeneity of vegetation composition.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart Valve

DISEASE(S): Infective Endocarditis

SUBMITTER: Daniel Martin  

LAB HEAD: Suneel Apte

PROVIDER: PXD019129 | Pride | 2020-07-29

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
24-1.msf Msf
37t-1.msf Msf
IEFractionated_2.mzML Mzml
IEFractionated_2.mzid.gz Mzid
IEFractionated_2.pride.mgf.gz Mgf
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Publications

Proteomics identifies a convergent innate response to infective endocarditis and extensive proteolysis in vegetation components.

Martin Daniel R DR   Witten James C JC   Tan Carmela D CD   Rodriguez E Rene ER   Blackstone Eugene H EH   Pettersson Gosta B GB   Seifert Deborah E DE   Willard Belinda B BB   Apte Suneel S SS  

JCI insight 20200723 14

Infective endocarditis is a life-threatening infection of heart valves and adjacent structures characterized by vegetations on valves and other endocardial surfaces, with tissue destruction and risk of embolization. We used high-resolution mass spectrometry to define the proteome of staphylococcal and non-staphylococcal vegetations and Terminal Amine Isotopic Labeling of Substrates (TAILS) to define their proteolytic landscapes. These approaches identified over 2000 human proteins in staphylococ  ...[more]

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