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AtLEGbeta / gamma protease specificity characterization by PICS

ABSTRACT: Arabidopsis thaliana Legumain (aka VPE) beta / gamma proteases were characterized for their cleavage specificity by PICS (Proteomic Identiﬁcation of protease Cleavage Sites).

INSTRUMENT(S): impact II

ORGANISM(S): Escherichia Coli Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Fatih Demir

LAB HEAD: Pitter Florian Huesgen

PROVIDER: PXD019220 | Pride | 2020-07-29

REPOSITORIES: pride

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			Action	DRS
	PICS_MQ_Search_Result.zip	Other
	PICS_Raw_Data.zip	Other

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Publications

Structural and functional studies of <i>Arabidopsis thaliana</i> legumain beta reveal isoform specific mechanisms of activation and substrate recognition.

Dall Elfriede E Zauner Florian B FB Soh Wai Tuck WT Demir Fatih F Dahms Sven O SO Cabrele Chiara C Huesgen Pitter F PF Brandstetter Hans H

The Journal of biological chemistry 20200721 37

The vacuolar cysteine protease legumain plays important functions in seed maturation and plant programmed cell death. Because of their dual protease and ligase activity, plant legumains have become of particular biotechnological interest, <i>e.g.</i> for the synthesis of cyclic peptides for drug design or for protein engineering. However, the molecular mechanisms behind their dual protease and ligase activities are still poorly understood, limiting their applications. Here, we present the crysta ...[more]

PMID: 32719006

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