Dataset Information

Variations of Metabolites and Proteome from Lonicera japonica Thunb. Buds Blossom to Flowers under UV Radiation

ABSTRACT: Lonicera japonica Thunb., known as Jin Yin Hua or Japanese honeysuckle, is an herbal medicine in Asian countries. Its flowers have been used as folk medicine for clinical practice or used as food or making healthy beverage for 1500 years in China. To investigate the molecular developmental processes from L. japonica buds to flowers under UV radiation, comparative proteomics analyses of buds and flowers were performed. Fifty-four differential proteins were identified including 42 increased proteins and 12 decreased proteins. The abundance of proteins related to glycolysis, TCA/organic acid transformation, major carbohydrate metabolism, oxidative pentose phosphate, stress, secondary metabolism, hormone, and mitochondrial electron transport were increased during flower opening process under UV radiation. Six metabolites were identified and relatively quantified by LC-MS/MS in L. japonica buds and flowers. The 1,1-diphenyl-2-picrylhydrazyl assay revealed that antioxidant activity of L. japonica buds was better than that of flowers. These results suggest that UV-B radiation could induce the production of endogenous ethylene in L. japonica buds, which facilitate the buds blossom and activate the antioxidant system. Additionally, the higher content of metabolites and antioxidant capability in L. japonica buds indicates that L. japonica buds stage might be the better harvest time compared to the flower.

INSTRUMENT(S): 4800 Plus MALDI TOF/TOF

ORGANISM(S): Lonicera Japonica

TISSUE(S): Plant Cell, Flower, Flower Bud

DISEASE(S): Disease Free

SUBMITTER: Wei Zhu

LAB HEAD: Wei Zhu

PROVIDER: PXD005324 | Pride | 2017-01-18

REPOSITORIES: Pride

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Variations of metabolites and proteome in Lonicera japonica Thunb. buds and flowers under UV radiation.

Zhu Wei W Zheng Wen W Hu Xingjiang X Xu Xiaobao X Zhang Lin L Tian Jingkui J

Biochimica et biophysica acta. Proteins and proteomics 20170110 4

Lonicera japonica Thunb., also known as Jin Yin Hua and Japanese honeysuckle, is used as a herbal medicine in Asian countries. Its flowers have been used in folk medicine in the clinic and in making food or healthy beverages for over 1500years in China. To investigate the molecular processes involved in L. japonica development from buds to flowers exposed to UV radiation, a comparative proteomics analysis was performed. Fifty-four proteins were identified as differentially expressed, including 4 ...[more]

PMID: 28087425

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