LC-MSMSMass Spectrometric detection of SARS-CoV-2 virus in scrapings of the epithelium of the nasopharynx of infected patients via Nucleocapsid N protein

ABSTRACT: Detection of viral RNA by PCR is currently the main diagnostic tool for COVID-191. The PCR-based test, however, shows limited sensitivity, especially at early and late stages of the disease development, and is relatively time consuming. Fast and reliable complementary methods for detecting the viral infection would be of help in the current pandemia conditions. Mass-spectrometry is one of such possibilities. We have developed a mass-spectrometry based method for the detection of the SARS CoV-2 virus in nasopharynx epithelial swabs, based on the detection of the viral nucleocapsid N protein. The N protein of the SARS-COV-2 virus, the most abundant protein in the virion, is the best candidate for mass-spectrometric detection of the infection, and MS-based detection of several peptides from the SARS-COoV-2 nucleoprotein has been reported earlier by the Sinz group. Our approach shows confident identification of the N protein in patient samples even with the lowest viral loads and a much simpler preparation procedure. Our main protocol consists of virus inactivation by heating and adding of isopropanol, and tryptic digestion of the proteins sedimented from the swabs followed by MS analysis. A set of unique peptides, produced as a result of proteolysis of the nucleocapsid phosphoprotein of SARS-CoV-2, is detected. The obtained results can further be used to create fast parallel mass-spectrometric approaches for the detection of the virus in the nasopharyngeal mucosa, saliva, sputum and other physiological fluids. Note: samples containing minor contamination of synthetic S peptides

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2

TISSUE(S): Nasopharynx

SUBMITTER: Alexander Brzhozovskiy  

LAB HEAD: Alexey Kononikhin

PROVIDER: PXD019648 | Pride | 2020-08-14

REPOSITORIES: Pride