Proteomics

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New insights into the role of peptide from Allomyrina dichotoma larvae in ameliorating lipid metabolism in vitro and in vivo

ABSTRACT: The aim of this study was to identify anti-obesity peptide from Allomyrina dichotoma (A. dichotoma) and investigate the biochemical signaling pathway. For that, A. dichotoma larvae was hydrolyzed enzymatically, and further purified by using tangential flow filtration and consecutive chromatographic method. Finally, anti-obesity peptide was obtained, and their sequences identified as Gln-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 prevented adipocyte differentiation in vitro and was further investigated the mechanism underlying the effects in vivo. Our results indicated that EIA10 reduced body weight gain, organ weight and adipose tissue volume. Glucose tolerance and insulin resistance in high fat diet-fed obese mice significantly improved after EIA10 administration for four weeks. In addition, EIA10 significantly decreased TC and LDL, increased HDL, improved lipid metabolism, and downregulated mRNA and protein expression of transcription factors implicated in lipid adipogenesis. Taken together our results suggest that EIA10 from possessed potential for the treatment and prevention of obesity.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Allomyrina

SUBMITTER: Byung-gyu kim  

LAB HEAD: Eun-Kyung Kim

PROVIDER: PXD019661 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity.

Bae Sung Mun SM   Fan Meiqi M   Choi Young-Jin YJ   Tang Yujiao Y   Jeong Gwanghui G   Myung Kyungjae K   Kim Byung-Gyu BG   Kim Eun-Kyung EK  

International journal of molecular sciences 20201112 22

The aim of this study was to identify an anti-obesity peptide from <i>Allomyrina dichotoma</i> and investigate the lipid metabolic mechanism. Enzymatically hydrolyzed <i>A. dichotoma</i> larvae were further separated using tangential flow filtration and consecutive chromatographic processes. Finally, an anti-obesity peptide that showed the highest inhibitory effect on lipid accumulation was obtained, and the sequence was Glu-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 decreased lipid aggr  ...[more]

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