Dataset Information

Molecular analyses of cotton (Gossypium hirsutum) identifies differential sensitivity to heat during stages of pollen development

ABSTRACT: Heatwaves resulting from global warming are a leading threat to plants because they specifically impair reproductive (gametophytic) development. We investigated pollen development using proteomics after exposure to heat (38/28 °C; day/night). Sampling enabled the identification of the impact of heat on gene products in tetrads (immediately post-meiosis), uninucleate and binucleate microspores (early mitosis), and mature pollen. A library consisting of 5257 G. hirsutum proteins was constructed using SWATH-MS, which led to quantification of 4501 proteins at the four distinct stages. Data analysis revealed that 880, 360, 307 and 166 proteins were differentially expressed when the tetrad, uninucleate, binucleate and mature pollen stages, respectively, were subjected to heat. Surprisingly, most of these DEPs were identified only at a single developmental stage, indicating translation of stage-specific proteins in response to heat. Especially, high numbers of differentially expressed proteins (DEPs) were identified in tetrads compared with the late developmental stages; these were mainly associated with protein processing in endoplasmic reticulum, oxidative phosphorylation, spliceosome, cytoskeletal response, chaperones and folding catalysts, carbon metabolism and membrane trafficking. We conclude that protein synthesis in tetrad cells responded acutely to heat by supplying cellular components that enable subsequent mitosis and structural changes in the haploid germ cells leading the mature pollen. We hypothesise that fewer DEPs at the later stages might reflect a lower sensitivity to high temperatures. Identifying the stage-specific proteins will lead to the identity of key heat-responsive genes and new genetic tools for improved resilience of all seed and fruit-based crops as climates keep warming.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Gossypium Hirsutum

TISSUE(S): Anther, Pollen, Leaf

SUBMITTER: Farhad Masoomi-Aladizgeh

LAB HEAD: Brian J. Atwell

PROVIDER: PXD019873 | Pride | 2021-12-01

REPOSITORIES: Pride

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Patterns of gene expression in pollen of cotton (Gossypium hirsutum) indicate downregulation as a feature of thermotolerance.

Masoomi-Aladizgeh Farhad F McKay Matthew J MJ Asar Yasmin Y Haynes Paul A PA Atwell Brian J BJ

The Plant journal : for cell and molecular biology 20211217 4

Reproductive performance in plants is impaired as maximum temperatures consistently approach 40°C. However, the timing of heatwaves critically affects their impact. We studied the molecular responses during pollen maturation in cotton to investigate the vulnerability to high temperature. Tetrads (TEs), uninucleate and binucleate microspores, and mature pollen were subjected to SWATH-MS and RNA-seq analyses after exposure to 38/28°C (day/night) for 5 days. The results indicated that molecular sig ...[more]

PMID: 34837283

Similar Datasets

Project description:Pollen development is central for plant reproduction and is assisted by changes of the transcriptome and the proteome. At the same time, pollen is largely heat sensitive, particularly at early developmental stages. To define the changes during development and the response of different stages to elevated temperatures we investigated the transcriptome and proteome of pollen at tetrad, post-meiotic and mature stage before and after application of elevated temperatures. We used Solanum lycopersicum as an economically important crop. We demonstrate that the number of transcripts declines from tetrad to mature stage, while the protein content shows the opposite trend. Comparison of the transcriptome and proteome led to the discovery of two translational modes assigned as direct translation – transcripts increased in a specific pollen stage lead to increased translation in the same stage – and delayed translation – transcripts are produced for translation in later stages. The response to elevated temperatures is with ~5% of all genes being upregulated comparable to general plant behavior. The alteration at proteome level marked the mature pollen as least responsive and the post-meiotic stages as most responsive with altered levels of 40% of the identified proteins. For a subset of the genes with direct translation we observed a downregulation of protein levels after heat stress, whereas some of the genes with delayed translation showed a shift from delayed to direct translation when heat stress is applied. Further, Hsps, proteasome subunits, ribosomal proteins as well as eukaryotic initiation factors are the most drastically affected groups. On the example of Hsps we document that the response at transcriptome and proteome levels in e.g. the post-meiotic and mature stage does not correlate. Moreover, while all stages respond to heat stress with regulation of transcript abundance of Hsps in a comparable manner, changes at proteome level are distinct. Thus, we demonstrate that the difference in heat stress response in different stages of pollen development is rather manifested by significant changes of protein than transcript abundance.

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