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NanoToxClass: Investigation of dose-dependent nanomaterial effects after short-term inhalation studies (STIS) with rats

ABSTRACT: In this study, rats were exposed to three different doses of nanomaterials (SiO2_7, TiO2_NM105) using short-term inhalations. Effects on exposure and recovery groups were compared using untargeted proteomics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Lung

SUBMITTER: Isabel Karkossa

LAB HEAD: Kristin Schubert

PROVIDER: PXD020178 | Pride | 2021-09-29

REPOSITORIES: Pride

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			Action	DRS
	CorrectionFactorsTMT_Inhalation.xlsx	Xlsx
	Labeling_Inhalation.xlsx	Xlsx
	NTC_Inhalation_10_f1.raw	Raw
	NTC_Inhalation_10_f2.raw	Raw
	NTC_Inhalation_1_f1.raw	Raw

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Publications

Nanomaterials induce different levels of oxidative stress, depending on the used model system: Comparison of in vitro and in vivo effects.

Karkossa Isabel I Bannuscher Anne A Hellack Bryan B Wohlleben Wendel W Laloy Julie J Stan Miruna S MS Dinischiotu Anca A Wiemann Martin M Luch Andreas A Haase Andrea A von Bergen Martin M Schubert Kristin K

The Science of the total environment 20210808

The immense diversity and constant development of nanomaterials (NMs) increase the need for a facilitated risk assessment, which requires knowledge of the modes of action (MoAs) of NMs. This necessitates a comprehensive data basis, which can be obtained using omics. Furthermore, the establishment of suitable in vitro test systems is essential to follow the 3R concept and to cope with the high number of NMs. In the present study, we aimed to compare NM effects in vitro and in vivo using a multi-o ...[more]

PMID: 34428663

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NanoToxClass: Investigation of dose-dependent nanomaterial effects after instillations with rats

Project description:In this study, rats were exposed to three different doses of nanomaterials (SiO2_15_Amino, SiO2_15_Unmod, SiO2_7, SiO2_40) using instillations. Effects on exposure and recovery groups were compared using untargeted proteomics.

2021-09-29 | PXD020184 | Pride

NanoToxClass: Investigation of dose-dependent nanomaterial effects in alveolar macrophages

Project description:In this study, alveolar macrophages (cell line NR8383) were treated with three different doses of nanomaterials (SiO2_15_Amino, SiO2_15_Unmod, SiO2_7, SiO2_40, TiO2_NM105) for 24 h. The effects were investigated using untargeted proteomics.

2021-09-29 | PXD020158 | Pride

NanoToxClass: Investigation of dose- and time-dependent nanomaterial effects in alveolar epithelial cells

Project description:In this study, alveolar epithelial cells (cell line RLE-6TN) were treated with three different doses of nanomaterials (SiO2_15_Amino, SiO2_15_Unmod, SiO2_7, SiO2_40, TiO2_NM105) for 6 h, 24 h, and 48 h, respectively. The effects were investigated using untargeted proteomics.

2021-09-29 | PXD020289 | Pride

The gut bacterium Extibacter muris produces secondary bile acids and influences liver physiology in gnotobiotic mice - Proteomics results

Project description:In this study, mice were colonized from birth on with the minimal microbiota Oligo-MM with or without the addition of Extibacter muris. Extibacter muris is known to metabolize cholic acid to deoxycholic acid via 7α-dehydroxylation. The impact of Extibacter muris colonization on the liver proteome of mice under different diets was investigated using untargeted proteomics.

2021-01-04 | PXD019973 | Pride

Maternal titanium dioxide nanomaterial inhalation exposure compromises placental hemodynamics.

Project description:The fetal consequences of gestational engineered nanomaterial (ENM) exposure are unclear. The placenta is a barrier protecting the fetus and allowing transfer of substances from the maternal circulation. The purpose of this study was to determine the effects of maternal pulmonary titanium dioxide nanoparticle (nano-TiO2) exposure on the placenta and umbilical vascular reactivity. We hypothesized that pulmonary nano-TiO2 inhalation exposure increases placental vascular resistance and impairs umbilical vascular responsiveness. Pregnant Sprague-Dawley rats were exposed via whole-body inhalation to nano-TiO2 with an aerodynamic diameter of 188 ± 0.36 nm. On gestational day (GD) 11, rats began inhalation exposures (6 h/exposure). Daily lung deposition was 87.5 ± 2.7 μg. Animals were exposed for 6 days for a cumulative lung burden of 525 ± 16 μg. On GD 20, placentas, umbilical artery and vein were isolated, cannulated, and treated with acetylcholine (ACh), angiotensin II (ANGII), S-nitroso-N-acetyl-DL-penicillamine (SNAP), or calcium-free superfusate (Ca2+-free). Mean outflow pressure was measured in placental units. ACh increased outflow pressure to 53 ± 5 mmHg in sham-controls but only to 35 ± 4 mmHg in exposed subjects. ANGII decreased outflow pressure in placentas from exposed animals (17 ± 7 mmHg) compared to sham-controls (31 ± 6 mmHg). Ca2+-free superfusate yielded maximal outflow pressures in sham-control (63 ± 5 mmHg) and exposed (30 ± 10 mmHg) rats. Umbilical artery endothelium-dependent dilation was decreased in nano-TiO2 exposed fetuses (30 ± 9%) compared to sham-controls (58 ± 6%), but ANGII sensitivity was increased (-79 ± 20% vs -36 ± 10%). These results indicate that maternal gestational pulmonary nano-TiO2 exposure increases placental vascular resistance and impairs umbilical vascular reactivity.

| S-EPMC6422339 | biostudies-literature

Uterine microvascular sensitivity to nanomaterial inhalation: An in vivo assessment.

Project description:With the tremendous number and diverse applications of engineered nanomaterials incorporated in daily human activity, exposure can no longer be solely confined to occupational exposures of healthy male models. Cardiovascular and endothelial cell dysfunction have been established using in vitro and in situ preparations, but the translation to intact in vivo models is limited. Intravital microscopy has been used extensively to understand microvascular physiology while maintaining in vivo neurogenic, humoral, and myogenic control. However, a tissue specific model to assess the influences of nanomaterial exposure on female reproductive health has not been fully elucidated. Female Sprague Dawley (SD) rats were exposed to nano-TiO2 aerosols (171 ± 6 nm, 10.1 ± 0.39 mg/m(3), 5h) 24-hours prior to experimentation, leading to a calculated deposition of 42.0 ± 1.65 ?g. After verifying estrus status, vital signs were monitored and the right horn of the uterus was exteriorized, gently secured over an optical pedestal, and enclosed in a warmed tissue bath using intravital microscopy techniques. After equilibration, significantly higher leukocyte-endothelium interactions were recorded in the exposed group. Arteriolar responsiveness was assessed using ionophoretically applied agents: muscarinic agonist acetylcholine (0.025 M; ACh; 20, 40, 100, and 200 nA), and nitric oxide donor sodium nitroprusside (0.05 M; SNP; 20, 40, and 100 nA), or adrenergic agonist phenylephrine (0.05 M; PE; 20, 40, and 100 nA) using glass micropipettes. Passive diameter was established by tissue superfusion with 10(-4)M adenosine. Similar to male counterparts, female SD rats present systemic microvascular dysfunction; however the ramifications associated with female health and reproduction have yet to be elucidated.

| S-EPMC4620930 | biostudies-literature

Gene expression profiles in rat lung following intratracheal instillation with short size single-wall and multi-wall carbon nanotubes

Project description:To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling to discuss the differences of the pulmonary and pleural Groups of nine-week-old male Wistar rats (n = 4 per group/ time point) were intratracheally instilled with short SWCNTs and MWCNTs suspended in 0.4 mL of 10mg/mL bovine serum albumin (BSA) as a single injection, respectively. Control groups received 10mg/

2015-11-19 | E-GEOD-75148 | biostudies-arrayexpress

nanomaterial toxicity to Pseudomonas

Project description:a comparison of the mechanisms of toxicity of zinc oxide nanomaterials and zinc ions to the bacterium Pseudomonas putida

| PRJEB9788 | ENA

Multi-parametric profiling of a large panel of engineered nanomaterials (ENM) points to surface chemistry as a key determinant of nanomaterial effects

Project description:Monocytic, cancer derived THP-1 cells were exposed to 31 different engineered nanomaterials (ENMs) with different core chemistries and surface modifications for 24 hours.

2020-12-01 | GSE148705 | GEO

Systems biology approaches reveal low-dose effects of nanoparticles

Project description:Here, we show that systems biology approaches can uncover mechanisms underlying cellular responses to nanomaterials. Using RNA Seq, we found that cationic nanoparticles are capable of triggering down-regulation of cell cycle-related genes in primary human bronchial epithelial cells at doses that do not elicit acute cytotoxicity. Bioinformatics analyses implicated NF-kappaB as a putative transcriptional regulator and functional assays confirmed that cationic nanoparticles caused NF-kappaB-dependent cell cycle arrest. Our study demonstrates the feasibility of applying systems biology tools to assess cellular responses to nanomaterials, not least at low doses.

2015-01-17 | E-MTAB-2397 | biostudies-arrayexpress