Dataset Information

Optimized Workflow for In-depth N-glycosylation Analysis of 2D and 3D Breast Cancer Cell Cultures and Xenografted Tumors

ABSTRACT: Native intact N-glycopeptides analysis can access to a lot of useful information including N-glycosites, N-glycans and N-glycoproteins of original complex samples. The traditional sample preprocessing method is to analyze the tryptic intact N-glycopeptides enriched using hydrophilic materials or some new materials by LC-MS/MS. However, the number of identified intact N-glycopeptides was very limited using existing methods. Herein, we systematically compared the different analytical methods, including the use of different sources of trypsin, combinations of different proteases and different enrichment materials.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yong Zhang

LAB HEAD: Yong Zhang

PROVIDER: PXD020254 | Pride | 2020-12-14

REPOSITORIES: Pride

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Publications

In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.

Mao Yonghong Y Zhao Yang Y Zhang Yong Y Yang Hao H

PloS one 20201210 12

Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples. The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection. However, the number of identified intact N-glycopeptides rem ...[more]

PMID: 33301522

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	2D-Proteomics_B_R1.raw	Raw
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