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Proteomic comparison of HliD and Ycf39 protein abundances in FLAG-tagged chlorophyll synthase co-immunoprecipitation assays of control and CrtR/CruF (carotenoid biosynthesis) knockout strains of Synechocystis sp. PCC 6803.

ABSTRACT: Chlorophyll a synthase (ChlG) is the enzyme responsible for attaching either a geranygeranyl or phytyl group to the chlorophyllide macrocycle during chlorophyll biosynthesis. Previous co-immunoprecipitation assays using FLAG-tagged ChlG identified the high light inducible protein HliD and the photosystem II assembly factor Ycf39 among the interaction partners. Non-protein components of this complex are the carotenoids zeaxanthin and myxoxanthophyll. To elucidate the function of these carotenoids in the association of ChlG/HliD/Ycf39, knockout strains of Synechocystis were produced to disrupt carotenoid biosynthesis. The abundances of HliD and Ycf39 relative to FLAG-tagged ChlG were compared in the different strains by proteomic analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Synechocystis Sp. (strain Pcc 6803 / Kazusa)

SUBMITTER: Philip Jackson

LAB HEAD: Andrew Hitchcock

PROVIDER: PXD020471 | Pride | 2022-08-18

REPOSITORIES: Pride

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			Action	DRS
	20190326_PJ_MP_all-expts.txt.zip	Txt
	20190326_PJ_MP_control1.raw	Raw
	20190326_PJ_MP_control2.raw	Raw
	20190326_PJ_MP_control3.raw	Raw
	20190326_PJ_MP_deltaCrtR1.raw	Raw

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Dataset Information

Proteomic comparison of HliD and Ycf39 protein abundances in FLAG-tagged chlorophyll synthase co-immunoprecipitation assays of control and CrtR/CruF (carotenoid biosynthesis) knockout strains of Synechocystis sp. PCC 6803.

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