Proteomics

Dataset Information

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Synthesis of chlorophyll-binding proteins in a fully-segregated ∆ycf54 strain of the cyanobacterium Synechocystis PCC 6803


ABSTRACT: The MS analysis is part of a wider study into chlorophyll biosynthesis. There is previous evidence, from both pulldown and CN-PAGE/immunoblot analyses that the enzyme Mg-protoporphyrin IX methylester cyclase (Cyc1), which catalyses the formation of the the fifth (E) ring in chlorophyll, associates with a protein of unknown function called Ycf54. There is also evidence for the association of Cyc1 with other enzymes in the chlorophyll biosynthetic pathway, eg. POR, ChlP and DVR. In the MS part of this study, FLAG-tagged Cyc1 was used to capture proteins from a Synechocystis cell lysate, with a comparison of FLAG-Cyc1 strains against wild-type and Ycf54 deletion backgrounds. MS-based quantification of the amounts of POR, ChlP and DVR relative to the Cyc1 bait supported evidence by immunoblotting of a significant reduction in these co-isolated proteins in the Ycf54 deletion strain.

INSTRUMENT(S): maXis, Q Exactive

ORGANISM(S): Synechocystis Sp. (strain Pcc 6803 / Kazusa)

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Philip Jackson  

LAB HEAD: Christopher Neil Hunter

PROVIDER: PXD003149 | Pride | 2016-04-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20150929_SH_I1.mgf Mgf
20150929_SH_I1.raw Raw
20150929_SH_I2.mgf Mgf
20150929_SH_I2.raw Raw
20150929_SH_I3.mgf Mgf
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Publications

Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803.

Hollingshead Sarah S   Kopečná Jana J   Armstrong David R DR   Bučinská Lenka L   Jackson Philip J PJ   Chen Guangyu E GE   Dickman Mark J MJ   Williamson Michael P MP   Sobotka Roman R   Hunter C Neil CN  

Frontiers in plant science 20160317


In the chlorophyll (Chl) biosynthesis pathway the formation of protochlorophyllide is catalyzed by Mg-protoporphyrin IX methyl ester (MgPME) cyclase. The Ycf54 protein was recently shown to form a complex with another component of the oxidative cyclase, Sll1214 (CycI), and partial inactivation of the ycf54 gene leads to Chl deficiency in cyanobacteria and plants. The exact function of the Ycf54 is not known, however, and further progress depends on construction and characterization of a mutant c  ...[more]

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