ABSTRACT: Here we provide a label-free interactome of PDE2A2 tagged with a Strep-tag II epitope at the carboxyl terminal in HT-4 mouse neuroblastoma cells.
Project description:Hemorrhagic transformation (HT), which occurs with or without reperfusion treatments (thrombolysis and/or thrombectomy), deteriorates the outcomes of ischemic stroke patients. It is essential to find clinically reliable biomarkers that can predict HT. In this study, we screened for potential serum biomarkers from an existing blood bank and database with 243 suspected acute ischemic stroke (AIS) patients. A total of 37 patients were enrolled, who were diagnosed as AIS but did not receive reperfusion treatment. They were divided into two groups based on whether they accompanied with HT or not (5 HT and 32 non-HT). Serum samples were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and compared under NCBInr database. A total of 647 proteins in sera samples were captured and the levels of 17 proteins (12 up-regulated and 5 down-regulated) were significantly different. These differentially expressed proteins were further categorized with Gene Ontology functional classification annotation and Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis into biological processes. Further protein-protein interaction analysis using String database discovered that, among the differential expressed proteins, 10 pairs of proteins were found to have crosstalk connections, which may have direct (physical) and indirect (functional) interactions for the development of HT. Our findings suggest that these differentially expressed proteins could serve as potential biomarkers for predicting HT after ischemic stroke.
Project description:This project demonstrates a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for identifying in a systems-wide manner bone fide substrates of βTrCP
Project description:This project demonstrates a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for identifying in a systems-wide manner bone fide substrates of βTrCP
Project description:Influenza A viruses (IAVs) are respiratory pathogens that represent a global concern due to their ability to cause seasonal epidemics and sporadic pandemics in the human population. Thus, a comprehensive understanding of IAV biology is essential in order to design effective antivirals. It is well established that entry of most IAVs begins through binding to cell surface sialic acid, however the identity of receptors that mediate virus internalization remains unclear. Here, we performed TurboID-based proximity labelling centered on epsin 1, a protein required for clathrin-mediated endocytosis of IAV, to uncover internalization receptors. By performing affinity-based purification of biotinylated proteins coupled to mass spectrometry, we identified 34 proteins proximal to epsin 1 during IAV infection. Therein we found 11 high-confidence STRING interactors for epsin 1 and 3 putative pro-viral host factors for IAV infection, validating our approach.
Project description:The purpose of this project is to examine the effects of rootstocks on the gene expression patterns in scions of apple trees. Gene expression patterns were examined in the Gala variety grafted onto seven different, commonly used rootstocks. These trees were grown in the greenhouse to limit environmental effects. Also, gene expression profiles were examined in three different varieties (Ambrosia, Melrose,and Gala) grafted onto B.9 rootstocks grown in the field. Each sample is a pool of RNA from two different trees. RNA samples were isolated from 0.5 g of actively growing shoot tips, including leaf and stem tissues.
Project description:This project aims to identify and differentiate the interaction partners of Axin1 isoforms including 1 wild type and 3 different mutants (L106R, L106R_F119R, deltaRGS).
Project description:This project aims to identify and differentiate the interaction partners of Axin1 isoforms including 1 wild type and 3 different mutants (L106R, L106R_F119R, deltaRGS)
2022-03-01 | PXD001822 | Pride
Project description:Strep. gordonii and Oral Biofilm Formation
| PRJNA882118 | ENA
Project description:Strep. gordonii and Oral Biofilm Formation