Dataset Information

Palaeoproteomic analysis of Pleistocene cave hyenas from east Asia

ABSTRACT: Spotted hyena (Crocuta crocuta) is the only extant species of the genus Crocuta, which once occupied a much wider range during the Pliocene and Pleistocene. However, its origin and evolutionary history is somewhat contentious due to discordances being found between morphological, nuclear, and mitochondrial data. Due to the limited molecular data from east Asian Crocuta, and the difficulty of extracting ancient DNA from this area, here we present proteomic analysis of cave hyenas from three locations in northern China. This marks the first proteomic data generated from cave hyenas, adding new molecular data to the east Asian populations. Phylogenetic analysis based on these protein sequences reveals two different groups of cave hyenas in east Asia, one of which could not be distinguished from modern spotted hyenas from northern Africa, tentatively the result of previously suggested gene flow between these lineages. With developments of instrumentation and analytical methods, proteomics holds promising potential for the phylogenetic reconstruction of ancient fauna previously thought to be unreachable using ancient DNA.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Crocuta

TISSUE(S): Bone Tissue, Bone, Tooth, Dentine

SUBMITTER: Huiyun Rao

LAB HEAD: Huiyun Rao

PROVIDER: PXD020530 | Pride | 2020-10-19

REPOSITORIES: Pride

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Palaeoproteomic analysis of Pleistocene cave hyenas from east Asia.

Rao Huiyun H Yang Yimin Y Liu Jinyi J Westbury Michael V MV Zhang Chi C Shao Qingfeng Q

Scientific reports 20201007 1

The spotted hyena (Crocuta crocuta) is the only extant species of the genus Crocuta, which once occupied a much wider range during the Pliocene and Pleistocene. However, its origin and evolutionary history is somewhat contentious due to discordances between morphological, nuclear, and mitochondrial data. Due to the limited molecular data from east Asian Crocuta, also known as cave hyena, and the difficulty of extracting ancient DNA from this area, here we present proteomic analysis of cave hyena ...[more]

PMID: 33028848

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			Action	DRS
	06SPE.mgf	Mgf
	06SPE.raw	Raw
	06SPT.mgf	Mgf
	06SPT.raw	Raw
	HZDPE.mgf	Mgf