Dataset Information

Barley root proteome and metabolome in response to cytokinin

ABSTRACT: Cytokinin is a phytohormone involved in the regulation of diverse developmental and physiological processes in plants. Its potential for biotechnology and development of high-yield and more resilient plants has been recognized, but the molecular mechanisms behind its action are far from understood. In this report, the roots of barley seedling were explored as a new tool to reveal as yet unknown cytokinin-responsive proteins. Significant differences were reproducibly observed for 176 proteins, and at least some of the revealed cytokinin-responsive pathways were confirmed in metabolome analysis, including alterations in phenylpropanoid pathway, amino acid biosynthesis or ROS metabolism. Bioinformatics analysis indicated a significant overlap between cytokinin response and response to abiotic stress. This was confirmed by comparing proteome and metabolome profiles in response to drought, salinity or a period of temperature stress. The results illustrate complex abiotic stress response in the early development of model crop plant and confirm an extensive crosstalk between plant hormone cytokinin and response to temperature stimuli, water availability or salinity.

INSTRUMENT(S): maXis

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Root

SUBMITTER: Martin Cerny

LAB HEAD: Martin Cerny

PROVIDER: PXD020627 | Pride | 2020-10-06

REPOSITORIES: Pride

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Publications

Barley Root Proteome and Metabolome in Response to Cytokinin and Abiotic Stimuli.

Berka Miroslav M Luklová Markéta M Dufková Hana H Berková Veronika V Novák Jan J Saiz-Fernández Iñigo I Rashotte Aaron M AM Brzobohatý Břetislav B Černý Martin M

Frontiers in plant science 20201028

Cytokinin is a phytohormone involved in the regulation of diverse developmental and physiological processes in plants. Its potential in biotechnology and for development of higher-yield and more resilient plants has been recognized, yet the molecular mechanisms behind its action are far from understood. In this report, the roots of barley seedlings were explored as a new source to reveal as yet unknown cytokinin-responsive proteins for crop improvement. Here we found significant differences repr ...[more]

PMID: 33250914

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			Action	DRS
	Barley_AllIDs.xlsx	Xlsx
	Peptides-list.xlsx	Xlsx
	Proteins-list.xlsx	Xlsx
	barley-30-1_GB8_01_9405.baf	Other
	barley-30-2_GC1_01_9431.baf	Other