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15N Stable-isotope-labeling in Arabidopsis (SILIA) quantitative MS analysis of the AtACINUS interactome

ABSTRACT: AtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modifications on AtACINUS. A homozygous 35S::AtACINUS-GFP/acinus-2 plant was selected for similar protein expression level to the endogenous AtACINUS protein of wild-type plants using a native α-AtACINUS antibody. We isolated putative AtACINUS interaction partners from young Arabidopsis seedlings using the native α-AtACINUS antibody. Plants expressing TAP-GFP under 35S promoter were used as controls.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Shouling Xu

LAB HEAD: SHOULING XU

PROVIDER: PXD020700 | Pride | 2020-12-02

REPOSITORIES: Pride

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			Action	DRS
	DATASETSUMMARY.xlsx	Xlsx
	V20121101-24.pep.xml	Pepxml
	V20121101-24.raw	Raw
	V20121101-25.pep.xml	Pepxml
	V20121101-25.raw	Raw

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