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Relative quantification of 15N labeled acinus-2 pinin-1 and 14N labeled Col through Data Dependent Acquisition to obtain peptide information for targeted Parallel Reaction Monitoring experiment

ABSTRACT: To examine the properties of 14N and 15N labeled peptides using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer we first set up this DDA experiment. The purpose of this experiment was to choose peptides that are good candidates for targeted quantification. The retention times, mass, and charge from 42 candidate peptides were acquired for targeted quantification.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Shouling Xu

LAB HEAD: Shouling Xu

PROVIDER: PXD030098 | Pride | 2022-06-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Q20210927_15.pep.xml	Pepxml
	Q20210927_15.raw	Raw
	Q20210927_15_AR_ap_col_N15_N14N15_A_Quantification.txt	Txt
	Q20210927_15_A_peptides.csv	Csv
	description.xlsx	Xlsx

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Publications

Application of Parallel Reaction Monitoring in 15N Labeled Samples for Quantification.

Reyes Andres V AV Shrestha Ruben R Baker Peter R PR Chalkley Robert J RJ Xu Shou-Ling SL

Frontiers in plant science 20220503

Accurate relative quantification is critical in proteomic studies. The incorporation of stable isotope 15N to plant-expressed proteins in vivo is a powerful tool for accurate quantification with a major advantage of reducing preparative and analytical variabilities. However, 15N labeling quantification has several challenges. Less identifications are often observed in the heavy-labeled samples because of incomplete labeling, resulting in missing values in reciprocal ...[more]

PMID: 35592564

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