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Using targeted quantification (PRM) to quantify proteins between wild type Col and acinus-2 pinin-1 mutant with 15N metabolic labeling

ABSTRACT: ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeling. Some of these altered proteins were subjected to targeted quantification.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Shouling Xu

LAB HEAD: Shouling Xu

PROVIDER: PXD030096 | Pride | 2022-04-19

REPOSITORIES: Pride

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			Action	DRS
	Description_of_raw_data_Targeted.xlsx	Xlsx
	Q20200409_02.pep.xml	Pepxml
	Q20200409_02.raw	Raw
	Q20200409_04.pep.xml	Pepxml
	Q20200409_04.raw	Raw

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Quantitative proteomic comparison between wild type Col and acinus-2 pinin-1 mutant using 15N metabolic labeling

Project description:ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeling.

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